{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:06:54Z","timestamp":1753870014644,"version":"3.41.2"},"reference-count":93,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T00:00:00Z","timestamp":1722384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["#204747"],"award-info":[{"award-number":["#204747"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Background: Human macrophages generate antimicrobial reactive nitrogen species in response to infection by <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (Mtb). Exposure to these redox\u2010reactive compounds induces stress response in Mtb, which can affect posttranslational modifications (PTM). Methods: Here, we present the global analysis of the PTM acylation of Mtb proteins in response to a sublethal dose of nitrosative stress in the form of nitric oxide (NO) using label free quantification. Results: A total of 6437 acylation events were identified on 1496 Mtb proteins, and O\u2010acylation accounted for 92.2% of the events identified, while 7.8% were N\u2010acylation events. About 22% of the sites identified were found to be acylated by more than one acyl\u2010group. Furthermore, the abundance of each acyl\u2010group decreased as their molecular weight increased. Quantitative PTM analysis revealed differential abundance of acylation in proteins involved in stress response, iron ion homeostasis, growth, energy metabolism, and antimicrobial resistance (AMR) induced by nitrosative stress over time. Conclusions: The results reveal a potential role of Mtb protein acylation in the bacterial stress responses and AMR. To our knowledge, this is the first report on global O\u2010acylation profile of Mtb in response to NO. This will significantly improve our understanding of the changes in Mtb acylation under nitrosative stress, highly relevant for global health.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202300212","type":"journal-article","created":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T13:53:17Z","timestamp":1722433997000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Differential Abundance of Protein Acylation in <i>Mycobacterium tuberculosis<\/i> Under Exposure to Nitrosative Stress"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5302-0919","authenticated-orcid":false,"given":"Alemayehu Godana","family":"Birhanu","sequence":"first","affiliation":[{"name":"Institute of Biotechnology Addis Ababa University  Addis Ababa Ethiopia"},{"name":"Department of Microbiology University of Oslo  Nydalen Oslo 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