{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T04:01:16Z","timestamp":1776052876107,"version":"3.50.1"},"reference-count":64,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T00:00:00Z","timestamp":1724803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005632","name":"Narodowe Centrum Bada\u0144 i Rozwoju","doi-asserted-by":"publisher","award":["DWM\/V4\u2010KOREA\/2\/7\/2024"],"award-info":[{"award-number":["DWM\/V4\u2010KOREA\/2\/7\/2024"]}],"id":[{"id":"10.13039\/501100005632","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Pressure cycling technology (PCT) coupled with data\u2010independent sequential window acquisition of all theoretical mass spectra (SWATH\u2010MS) can be a powerful tool for identifying and quantifying biomarkers (e.g., proteins) in complex biological samples. Mouse models are frequently used in brain studies, including those focusing on different neurodevelopmental and psychiatric disorders. More and more pieces of evidence have suggested that sex\u2010related differences in the brain impact the rates, clinical manifestations, and therapy outcomes of these disorders. However, sex\u2010based differences in the proteomic profiles of mouse cerebella have not been widely investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>In this pilot study, we evaluate the applicability of coupling PCT sample preparation with microLC\u2010SWATH\u2010MS analysis to map and identify differences in the proteomes of two female and two male mice cerebellum samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified and quantified 174 proteins in mice cerebella. A comparison of the proteomic profiles revealed that the levels of 11 proteins in the female and male mice cerebella varied significantly.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Although this study utilizes a small sample, our results indicate that the studied male and female mice cerebella possessed differing proteome compositions, mainly with respect to energy metabolism processes.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202400001","type":"journal-article","created":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T05:59:00Z","timestamp":1724911140000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pressure Cycling Technology Combined With MicroLC\u2010SWATH Mass Spectrometry for the Analysis of Sex\u2010Related Differences Between Male and Female Cerebella: A Promising Approach to Investigating Proteomics Differences in Psychiatric and Neurodegenerative Diseases"],"prefix":"10.1002","volume":"18","author":[{"given":"Katarzyna","family":"Macur","sequence":"first","affiliation":[{"name":"Core Facility Laboratories, Intercollegiate Faculty of Biotechnology University of Gda\u0144sk and Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Roszkowska","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulina","family":"Czaplewska","sequence":"additional","affiliation":[{"name":"Core Facility Laboratories, Intercollegiate Faculty of Biotechnology University of Gda\u0144sk and Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Mi\u0119kus\u2010Purwin","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilona","family":"Klejbor","sequence":"additional","affiliation":[{"name":"Department of Anatomy Institute of Medical Sciences Jan Kochanowski University  Kielce Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janusz","family":"Mory\u015b","sequence":"additional","affiliation":[{"name":"Department of Normal Anatomy Pomeranian Medical University  Szczecin Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6468-1974","authenticated-orcid":false,"given":"Tomasz","family":"B\u0105czek","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Medical University of Gda\u0144sk  Gda\u0144sk Poland"},{"name":"Department of Nursing and Medical Rescue Institute of Health Sciences Pomeranian University in S\u0142upsk  S\u0142upsk Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4160"},{"key":"e_1_2_9_3_1","first-page":"1","article-title":"Fibrillar A\u03b2 Triggers Microglial Proteome Alterations and Dysfunction in Alzheimer Mouse Models","volume":"9","author":"Monasor L. S.","year":"2020","journal-title":"Elife"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.12.015"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13149"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2017.10.002"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23809"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.31887\/DCNS.2016.18.4\/jmarrocco"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9020313"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11797-3"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2015.00066"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12311-015-0715-3"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature19949"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24065350"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms16023537"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2019.08.032"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c00470"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.9b00662"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.7b00433"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac503952q"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201500161"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.5b01136"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr200805u"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3807"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201900253"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2013.03.023"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.O111.016717"},{"key":"e_1_2_9_28_1","unstructured":"Thermo Scientific.Pierce C18 Tips User Manual n.d."},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0294236"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1000"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0815-6"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2018.02.026"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-020-00384-6"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20178126"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20191091"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2016.12.022"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2018.10.025"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/brainsci8020033"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-022-04607-5"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.07.029"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.06.003"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00062.2017"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-015-0201-x"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/46224"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-00311-7"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2020.609517"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00031.2018"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1515\/bmc-2019-0001"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.200224"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24924"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.2152\/jmi.52.127"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-36209-5"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gal062"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.049684"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2020.148276"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20190041"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22126188"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2011.07189.x"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40291-018-0352-x"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23169212"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyt.2021.546801"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1159\/000460818"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2021.173895"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1038"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1040"}],"container-title":["PROTEOMICS \u2013 Clinical Applications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prca.202400001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T13:38:29Z","timestamp":1731591509000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prca.202400001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,28]]},"references-count":64,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/prca.202400001"],"URL":"https:\/\/doi.org\/10.1002\/prca.202400001","archive":["Portico"],"relation":{},"ISSN":["1862-8346","1862-8354"],"issn-type":[{"value":"1862-8346","type":"print"},{"value":"1862-8354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,28]]},"assertion":[{"value":"2024-01-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202400001"}}