{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T19:26:01Z","timestamp":1783625161086,"version":"3.55.0"},"reference-count":82,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T00:00:00Z","timestamp":1722643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Severe congenital neutropenia (SCN) is a raredisorder characterized by diminished neutrophil levels. Despite granulocytecolony\u2010stimulating factor (G\u2010CSF) treatment, SCN patients remain still prone tosevere infections, including periodontal disease\u2014a significant oral healthrisk. This study investigates the host proteome and metaproteome in saliva andgingival crevicular fluid (GCF) of G\u2010CSF\u2010treated patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>We used label\u2010free quantitative proteomics on saliva and GCF samples from SCN patients before (<jats:italic>n<\/jats:italic>\u00a0=\u00a010, mean age: 10.7\u00a0\u00b1\u00a06.6 years) and after a 6\u2010month oral hygiene intervention (<jats:italic>n<\/jats:italic>\u00a0=\u00a09,mean age: 11.6\u00a0\u00b1\u00a05.27 years), and from 12 healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We quantified 894 proteins in saliva (648 human,246 bacterial) and 756 proteins in GCF (493 human, 263 bacterial). Predominant bacterial genera included <jats:italic>Streptococcus<\/jats:italic>, <jats:italic>Veillonella<\/jats:italic>, <jats:italic>Selenomonas<\/jats:italic>, <jats:italic>Corynebacterium<\/jats:italic>, <jats:italic>Porphyromonas<\/jats:italic>, and <jats:italic>Prevotella<\/jats:italic>. SCN patients showed reduced antimicrobial peptides (AMPs) and elevated complement proteins compared tohealthy controls. Oral hygiene intervention improved oral epithelial conditionsand reduced both AMPs and complement proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>SCN patients have aunique proteomic profile with reduced AMPs and increased complement proteins, contributing to infection susceptibility. Oral hygiene intervention not onlyimproved oral health in SCN patients but also offers potential overall therapeuticbenefits.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202400064","type":"journal-article","created":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T14:05:22Z","timestamp":1722693922000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Protein Network Alterations in G\u2010CSF Treated Severe Congenital Neutropenia Patients and Beneficial Effects of Oral Health Intervention"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1183-5607","authenticated-orcid":false,"given":"Kai","family":"Bao","sequence":"first","affiliation":[{"name":"Division of Oral Health and Periodontology Department of Dental Medicine Karolinska Institutet  Huddinge 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