{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:07:26Z","timestamp":1773014846036,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T00:00:00Z","timestamp":1762128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T00:00:00Z","timestamp":1762128000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82560244\/H0906"],"award-info":[{"award-number":["82560244\/H0906"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The \u201cblend sign\u201d is a critical CT imaging marker for predicting hematoma expansion in intracerebral hemorrhage (ICH). This study aimed to elucidate its underlying pathological mechanisms by comparing proteomic profiles between hyperdense and hypodense regions within the hematoma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Hematoma samples from nine ICH patients exhibiting the blend sign were obtained via minimally invasive puncture. Isotope\u2010labeled proteomics and bioinformatic analyses were performed to identify differentially expressed proteins (DEPs), which were further validated by Western blotting and ELISA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 77 DEPs were identified, including 66 upregulated and 11 downregulated in hyperdense regions compared to hypodense areas. Functional enrichment analysis revealed significant involvement of inflammatory responses, apoptosis, oxidative stress, and metabolic dysregulation. Notably, cytochrome C, growth\u2010associated protein 43 (GAP43), and tau were markedly upregulated in hyperdense regions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The blend sign is associated with region\u2010specific molecular changes involving inflammation, apoptosis, and metabolic alterations. These findings provide mechanistic insights into hematoma heterogeneity and lay a foundation for future studies exploring their role in hematoma expansion and clinical outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/prca.70028","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T20:19:37Z","timestamp":1762201177000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Proteomic Insights Into the Pathogenesis of Intracerebral Hemorrhage: The Role of Blend Sign in Hematoma Expansion"],"prefix":"10.1002","volume":"20","author":[{"given":"Hang","family":"Hang","sequence":"first","affiliation":[{"name":"Emergency Department The Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou Province China"},{"name":"Beijing Institute of Brain Disorders Laboratory of Brain Disorders Ministry of Science and Technology Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanpeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Brain Disorders Laboratory of Brain Disorders Ministry of Science and Technology Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Likun","family":"Wang","sequence":"additional","affiliation":[{"name":"Emergency Department The Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linshan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Emergency Department The Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuiying","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Nursing Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baohui","family":"Xu","sequence":"additional","affiliation":[{"name":"Division of Vascular Surgery Department of Surgery Stanford University School of Medicine  Stanford California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4722-5634","authenticated-orcid":false,"given":"Heng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing Institute of Brain Disorders Laboratory of Brain Disorders Ministry of Science and Technology Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guofeng","family":"Wu","sequence":"additional","affiliation":[{"name":"Emergency Department The Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,3]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-023-00424-7"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12028\u2010022\u201001609\u2010w"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.319949"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.121.037591"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2020.00702"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrc.2020.10.029"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/strokeaha.115.009185"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12028\u2010020\u201001165\u20101"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40120\u2010021\u201000306\u20108"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.122.041246"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/1747493017694391"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.24133"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1752\u20100509\u20108\u2010s4\u2010s11"},{"issue":"4","key":"e_1_2_13_15_1","article-title":"Biomarkers of Parkinson's Disease: From Basic Research to Clinical Practice","volume":"15","author":"Ma Z.\u2010L.","year":"2024","journal-title":"Aging & Disease"},{"issue":"1","key":"e_1_2_13_16_1","first-page":"43","article-title":"Alzheimer's Disease Puzzle: Delving into Pathogenesis Hypotheses","volume":"15","author":"Nasb M.","year":"2024","journal-title":"Aging and Disease"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.57078"},{"key":"e_1_2_13_18_1","first-page":"1","article-title":"Mitochondrial\u2010Based Therapeutic Strategies for Intracerebral Hemorrhage","author":"Li X.","year":"2022","journal-title":"Translational Stroke Research"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.112749"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2023.1080344"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.847246"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974\u2010025\u201003373\u2010y"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13060493"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2020.615451"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.14853"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242417146"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0315333"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40001\u2010024\u201001842\u20103"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450117666160712092224"},{"issue":"2","key":"e_1_2_13_30_1","first-page":"560","article-title":"Plasma Neurofilament Light Chain Predicts Mortality and Long\u2010Term Neurological Outcomes in Patients With Intracerebral Hemorrhage","volume":"14","author":"Zheng P.","year":"2023","journal-title":"Aging and Disease"}],"container-title":["PROTEOMICS \u2013 Clinical Applications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prca.70028","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/prca.70028","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prca.70028","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:58:48Z","timestamp":1773010728000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prca.70028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,3]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/prca.70028"],"URL":"https:\/\/doi.org\/10.1002\/prca.70028","archive":["Portico"],"relation":{},"ISSN":["1862-8346","1862-8354"],"issn-type":[{"value":"1862-8346","type":"print"},{"value":"1862-8354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,3]]},"assertion":[{"value":"2025-02-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70028"}}