{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T02:53:59Z","timestamp":1780541639362,"version":"3.54.1"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T00:00:00Z","timestamp":1763769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T00:00:00Z","timestamp":1763769600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Polycystic ovary syndrome (PCOS) is a metabolic disorder affecting women of reproductive age, and its etiology remains unclear. Therefore, it is crucial to identify biomarkers of the metabolic disturbances in PCOS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of eight clinical PCOS samples and control group samples were analyzed using data\u2010independent acquisition (DIA) proteomics. Clinical data were used to identify protein biomarkers, and enzyme\u2010linked immunosorbent assay (ELISA) validation was performed on 27 PCOS and 23 control samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In the PCOS samples, a total of 114 differentially expressed proteins were identified, with 37 upregulated and 77 downregulated. Further biofunctional analysis using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways revealed two differentially expressed proteins, lactate dehydrogenase A (LDHA) and triosephosphate isomerase 1 (TPI1), both of which were significantly upregulated in clinical PCOS samples. LDHA and TPI1 are involved in the glycolysis\/gluconeogenesis metabolic pathway. Finally, ELISA was used to validate the high expression of LDHA and TPI1 in PCOS patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>DIA proteomics effectively identifies PCOS biomarkers. LDHA and TPI1 may serve as diagnostic biomarkers and could exert effects through glycolytic pathways.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/prca.70032","type":"journal-article","created":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T11:24:22Z","timestamp":1763810662000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Increased Serum Levels of LDHA and TPI1 in Patients With Polycystic Ovary Syndrome"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7227-3230","authenticated-orcid":false,"given":"Wenjing","family":"Wang","sequence":"first","affiliation":[{"name":"Prenatal Diagnostic Center Dongguan Maternal and Children Health Hospital  Dongguan Guangdong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tizhen","family":"Yan","sequence":"additional","affiliation":[{"name":"Prenatal Diagnostic Center Dongguan Maternal and Children Health Hospital  Dongguan Guangdong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manna","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Obstetrics &amp; Gynecolog Dongguan Maternal and Children Hospital  Dongguan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueliang","family":"Li","sequence":"additional","affiliation":[{"name":"Prenatal Diagnostic Center Dongguan Maternal and Children Health Hospital  Dongguan Guangdong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiwu","family":"Lou","sequence":"additional","affiliation":[{"name":"Prenatal Diagnostic Center Dongguan Maternal and Children Health Hospital  Dongguan Guangdong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez185"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10071505"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2019.04.006"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.3464"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh098"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.4158\/EP15748.DSC"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/14789450.2023.2275683"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471\u20100528.2008.02041.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112031"},{"issue":"5","key":"e_1_2_10_11_1","first-page":"1409","article-title":"Proteomics Analysis of Potential Serum Biomarkers for Insulin Resistance in Patients With Polycystic Ovary Syndrome","volume":"45","author":"Li L.","year":"2020","journal-title":"International Journal of Molecular Medicine"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010020\u20100406\u20102"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2013.1250"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14642"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/bab.2108"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr100008e"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12933\u2010019\u20100846\u20109"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/obr.12762"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2022.11.007"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr3000317"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.31425"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916\u2010023\u201002775\u20100"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.73890"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2024.1301099"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.130232"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.11.156"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/08916934.2024.2384889"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.12891\/ceog1779.2015"},{"key":"e_1_2_10_29_1","first-page":"1004","article-title":"Sex Hormone Binding Globulin, but Not Testosterone, Is Associated With the Metabolic Syndrome in Overweight and Obese Women With Polycystic Ovary Syndrome","volume":"36","author":"Moran L. J.","year":"2013","journal-title":"Journal of Endocrinological Investigation"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2022.956406"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12953\u2010024\u201000238\u20109"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky869"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1081"}],"container-title":["PROTEOMICS \u2013 Clinical Applications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prca.70032","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/prca.70032","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prca.70032","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:58:51Z","timestamp":1773010731000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prca.70032"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,22]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/prca.70032"],"URL":"https:\/\/doi.org\/10.1002\/prca.70032","archive":["Portico"],"relation":{},"ISSN":["1862-8346","1862-8354"],"issn-type":[{"value":"1862-8346","type":"print"},{"value":"1862-8354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,22]]},"assertion":[{"value":"2025-02-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70032"}}