{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T09:54:06Z","timestamp":1774605246059,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82371348"],"award-info":[{"award-number":["82371348"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Ischemic stroke (IS) is a severe neurological disease with limited treatment options. Subcutaneous adipose tissue\u2010derived small extracellular vesicles (SAT\u2010sEVs), which are readily accessible and abundant, have emerged as promising biomarkers and therapeutic agents in various diseases. The objective of this research is to uncover the roles and regulatory mechanisms of proteins and miRNAs contained within SAT\u2010sEVs in middle cerebral artery occlusion (MCAO) rats, aiming to discover innovative approaches and insights for IS treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Design<\/jats:title>\n                    <jats:p>To evaluate the therapeutic efficacy of SAT\u2010sEVs, we intravenously administered them to MCAO rats and assessed neurological function and cerebral infarction 24\u00a0h after SAT\u2010sEVs administration using behavioral scoring and TTC staining. To elucidate the potential mechanisms and ischemia\u2010induced alterations in SAT\u2010sEVs, we conducted integrated transcriptomic and proteomic analyses on vesicles isolated from both MCAO rats (24\u2010h post\u2010ischemia) and normal controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>SAT\u2010sEVs markedly alleviated neurological impairments and decreased the volume of cerebral infarcts in MCAO rats. Significant alterations were also observed in the miRNAs and proteins within SAT\u2010sEVs following ischemic injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Relevance<\/jats:title>\n                    <jats:p>This comprehensive analysis enhances our understanding of SAT\u2010sEVs\u2010mediated protective mechanisms and functional alterations in IS. It establishes a solid experimental basis for the potential clinical use of SAT\u2010sEVs in stroke rehabilitation and other related diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/prca.70039","type":"journal-article","created":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T08:00:03Z","timestamp":1769846403000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Proteomic and Transcriptomic Analysis of Adipose Tissue\u2010Derived Small Extracellular Vesicles in Ischemic Stroke"],"prefix":"10.1002","volume":"20","author":[{"given":"Zhichen","family":"Liao","sequence":"first","affiliation":[{"name":"School of Biological Science and Medical Engineering Beihang University  Beijing China"},{"name":"Department and Institute of Clinical Laboratory Xuanwu Hospital Capital Medical University  Beijing 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