{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T09:54:16Z","timestamp":1774605256895,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T00:00:00Z","timestamp":1773446400000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Coronavirus disease 2019 (COVID\u201019) has highlighted significant neurological complications in severe cases. Cerebrospinal fluid (CSF) proteomics could reveal biomarkers related to clinical outcome among critically ill patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Design<\/jats:title>\n                    <jats:p>We performed high\u2010resolution proteomic analyses of CSF samples from 29 intensive care unit (ICU) patients with severe COVID\u201019 and 19 controls. Differentially expressed proteins and associated pathways were identified through bioinformatic and statistical analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Proteomic analysis identified 488 significantly altered proteins between COVID\u201019 patients and controls. Proteins linked to coagulation, inflammation, and blood\u2010brain barrier dysfunction (e.g., SERPINC1, KNG1, PLG) were elevated in patients who survived ICU admission. Conversely, proteins associated with metabolic disruption, cellular stress, and neuroinflammation (e.g., FABP3, PDIA4) were upregulated in non\u2010survivors. Pathway enrichment analyses confirmed involvement of immune activation, inflammatory responses, and coagulation cascades.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and clinical relevance<\/jats:title>\n                    <jats:p>CSF proteomics in severe COVID\u201019 patients reveals potential biomarkers predictive of patient outcomes. These findings support the involvement of systemic inflammation and blood\u2010brain barrier disruption in COVID\u201019 pathophysiology, suggesting novel targets for personalized intervention strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/prca.70042","type":"journal-article","created":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T07:51:33Z","timestamp":1773474693000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Proteomic Analysis of Cerebrospinal Fluid from Severe COVID\u201019 Patients Reveals Prognostic Biomarkers Associated with Disease Outcome"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8672-7619","authenticated-orcid":false,"given":"Harry","family":"Alexopoulos","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Biophysics Faculty of Biology National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Samiotaki","sequence":"additional","affiliation":[{"name":"Institute For Bioinnovation BSRC \u201cA. Fleming\u201d  Vari Attica Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eirini","family":"Gkogkou","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Biophysics Faculty of Biology National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia","family":"Mavromati","sequence":"additional","affiliation":[{"name":"1st Department of Intensive Care, Evangelismos Hospital, Faculty of Medicine National and Kapodistrian University of Athens  Athens Greece"},{"name":"Department of Neurology General and Oncological Hospital of Kifisia  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kleopatra","family":"Bitzogli","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Faculty of Medicine National and Kapodistrian University of Athens"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgios","family":"Panagiotou","sequence":"additional","affiliation":[{"name":"Institute For Bioinnovation BSRC \u201cA. 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