{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T04:59:07Z","timestamp":1785301147154,"version":"3.55.0"},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and aims<\/jats:title>\n                    <jats:p>Non\u2010alcoholic Fatty Liver Disease (NAFLD) affects about a quarter of the world's population. Liver biopsy remains the gold standard for diagnosing the progressive form of NAFLD called Non\u2010alcoholic Steatohepatitis (NASH) but it is invasive, prone to sampling errors and observer variability, and impractical for widespread use. Fecal non\u2010invasive biomarkers have emerged as a new approach to diagnose, stage, and monitor NAFLD over time.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Proteomic analysis was performed using Liquid Chromatography\u2010Mass Spectrometry (LC\u2010MS\/MS) in fecal samples derived from mice fed with high fat and sugar diet representing NAFLD model and with the addition of 5 cycles of dextran\u2010sulphate in drinking water to induce a NASH.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Qualitative and quantitative different protein profiles resulted in a comparison between the three groups of mice. An enrichment analysis of differentially expressed proteins explores the molecular pathways involved. A selection of the most significant modulated proteins between groups was performed. The proteins Transthyretin, Kallikrein1 and Trefoil Factor 3 were validated by ELISA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our exploratory discovery study identified several proteins that, alone or in combination, enable a good separation between NASH, NAFLD and healthy mice, highlighting their potential as key targets for future research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/prca.70047","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T04:46:12Z","timestamp":1780116372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fecal Proteomics Suggest Potential Biomarkers for Non\u2010Alcoholic Fatty Liver Disease and Steatohepatitis"],"prefix":"10.1002","volume":"20","author":[{"given":"Anna","family":"Negroni","sequence":"first","affiliation":[{"name":"Division of Biotechnologies, ENEA Casaccia Research Center  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7141-1817","authenticated-orcid":false,"given":"Luisa","family":"Pieroni","sequence":"additional","affiliation":[{"name":"Departmental Faculty of Medicine and Surgery UniCamillus\u00a0\u2010 Saint Camillus International University of Health Sciences  Rome Italy"},{"name":"IRCCS San Camillo Hospital  Venice Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noemi","family":"Fiaschini","sequence":"additional","affiliation":[{"name":"Nanofaber S.r.l.  Rome Italy"},{"name":"Department For Sustainability Division Sustainable Materials, ENEA Casaccia Research Centre  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Palone","sequence":"additional","affiliation":[{"name":"Division of Biotechnologies, ENEA Casaccia Research Center  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0912-9287","authenticated-orcid":false,"given":"Mariateresa","family":"Mancuso","sequence":"additional","affiliation":[{"name":"Division of Biotechnologies, ENEA Casaccia Research Center  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4203-0163","authenticated-orcid":false,"given":"Roberta","family":"Vitali","sequence":"additional","affiliation":[{"name":"Division of Biotechnologies, ENEA Casaccia Research Center  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,29]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.32861"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.10.033"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13098\u2010020\u201000570\u2010y"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/3920196"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhepr.2019.07.002"},{"key":"e_1_2_12_7_1","first-page":"S1","article-title":"Type 2 Diabetes Mellitus and Non\u2010alcoholic Fatty Liver Disease: A Systematic Review and Meta\u2010analysis","volume":"10","author":"Atan N. 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