{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:59:52Z","timestamp":1790341192943,"version":"4.1.0"},"reference-count":78,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000233","name":"Genome British Columbia","doi-asserted-by":"publisher","award":["374PRO"],"award-info":[{"award-number":["374PRO"]}],"id":[{"id":"10.13039\/501100000233","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PJT\u2010506322"],"award-info":[{"award-number":["PJT\u2010506322"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      High\u2010grade serous ovarian carcinoma (HGSOC) frequently recurs after platinum\u2013taxane therapy, yet recurrence\u2010associated changes within ascites remain unclear. We performed cell\u2010type\u2010resolved quantitative proteomics on paired ascites samples collected at primary diagnosis and at disease recurrence from three HGSOC patients, profiling unsorted cells alongside matched CD45\n                      <jats:sup>\u2212<\/jats:sup>\n                      tumor\u2010enriched and CD45\n                      <jats:sup>+<\/jats:sup>\n                      immune\u2010enriched fractions. Conventional proteomic label\u2010free quantitative (LFQ) analysis yielded limited significant proteins due to inter\u2010patient heterogeneity. To address this, we applied Gene Set Enrichment Analysis (GSEA) to identify coordinated pathway\u2010level alterations associated with recurrence. Across all cellular compartments, recurrent samples exhibited consistent downregulation of interferon\u2010\u03b1\/\u03b3\u2013associated pathways alongside enrichment of oxidative phosphorylation and stress\u2010adaptive metabolic programs. In the CD45\n                      <jats:sup>+<\/jats:sup>\n                      immune compartment, these changes were accompanied by enhanced IL\u20102\/STAT5 signaling and reduced antigen\u2010presentation pathways. Together, these findings suggest that recurrent ascites is characterized by a shift toward oxidative metabolism and a more immunosuppressive microenvironment.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/prca.70053","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T12:14:33Z","timestamp":1787055273000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cell\u2010Type\u2010Resolved Proteomics Reveals Distinct Immune and Metabolic Programs Between Primary and Recurrent Ascites in HGSOC"],"prefix":"10.1002","volume":"20","author":[{"given":"Bo","family":"Ren","sequence":"first","affiliation":[{"name":"School of Molecular Life Sciences University of Victoria  Victoria British Columbia Canada"},{"name":"Genome BC Proteomics Centre University of Victoria  Victoria British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8947-7793","authenticated-orcid":false,"given":"Kenneth","family":"Weke","sequence":"additional","affiliation":[{"name":"Genome BC Proteomics Centre University of Victoria  Victoria British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4309-6586","authenticated-orcid":false,"given":"Darryl","family":"Hardie","sequence":"additional","affiliation":[{"name":"Genome BC Proteomics Centre University of Victoria  Victoria British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"MacPherson","sequence":"additional","affiliation":[{"name":"Deeley Research Centre BC Cancer Research Institute  Victoria British Columbia Canada"},{"name":"Basic and Translational Research BC Cancer Research Institute  Victoria British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier A","family":"Alfaro","sequence":"additional","affiliation":[{"name":"Department of Biochemistry &amp; Microbiology University of Calgary  Calgary Alberta Canada"},{"name":"Riddell Centre for Cancer Immunotherapy Arnie Charbonneau Cancer Institute Cumming School of Medicine University of Calgary  Calgary Alberta Canada"},{"name":"Department of Computer Science University of Calgary  Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5624-3541","authenticated-orcid":false,"given":"Julian J","family":"Lum","sequence":"additional","affiliation":[{"name":"School of Molecular Life Sciences University of Victoria  Victoria British Columbia Canada"},{"name":"Deeley Research Centre BC Cancer Research Institute  Victoria British Columbia Canada"},{"name":"Basic and Translational Research BC Cancer Research Institute  Victoria British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8045-8200","authenticated-orcid":false,"given":"David R","family":"Goodlett","sequence":"additional","affiliation":[{"name":"School of Molecular Life Sciences University of Victoria  Victoria British Columbia Canada"},{"name":"Genome BC Proteomics Centre University of Victoria  Victoria British Columbia 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