{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:53:18Z","timestamp":1791237198225,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,1,31]],"date-time":"2012-01-31T00:00:00Z","timestamp":1327968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The polypeptide hormone Islet Amyloid Polypeptide (IAPP, amylin) is responsible for islet amyloid formation in type\u20102 diabetes and in islet cell transplants, where it may contribute to graft failure. Human IAPP is extremely amyloidogenic and fewer inhibitors of IAPP amyloid formation have been reported than for the Alzheimer's A\u03b2 peptide or for \u03b1\u2010synuclein. The ability of a set of hydroxyflavones to inhibit IAPP amyloid formation was tested. Fluorescence detected thioflavin\u2010T\u2010binding assays are the most popular methods for measuring the kinetics of amyloid formation and for screening potential inhibitors; however, we show that they can lead to false positives with hydroxyflavones. Several of the compounds inhibit thioflavin\u2010T fluorescence, but not amyloid formation; a result which highlights the hazards of relying solely on thioflavin\u2010T assays to screen potential inhibitors. Transmission electron microscopy (TEM) and right\u2010angle light scattering show that Morin hydrate (2\u2032,3,4\u2032,5,7\u2010Pentahydroxyflavone) inhibits amyloid formation by human IAPP and disaggregates preformed IAPP amyloid fibers. In contrast, Myricetin, Kaempferol, and Quercetin, which differ only in hydroxyl groups on the B\u2010ring, are not effective inhibitors. Morin hydrate represents a new type of IAPP amyloid inhibitor and the results with the other compounds highlight the importance of the substitution pattern on the B\u2010ring.<\/jats:p>","DOI":"10.1002\/pro.2023","type":"journal-article","created":{"date-parts":[[2012,1,11]],"date-time":"2012-01-11T18:07:44Z","timestamp":1326305264000},"page":"373-382","source":"Crossref","is-referenced-by-count":118,"title":["Morin hydrate inhibits amyloid formation by islet amyloid polypeptide and disaggregates amyloid fibers"],"prefix":"10.1002","volume":"21","author":[{"given":"Harris","family":"Noor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel P.","family":"Raleigh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,1,31]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.3109\/10408369409084679"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1104-1054"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.75.101304.123901"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0507471103"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.65"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2008.151"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ar8000475"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja072157y"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02442587"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0442hyp"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200705372"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2005.00318.x"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja1046186"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1437"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05611.x"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0600749"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddl210"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi100939a"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2011.07.008"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0910723107"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811827106"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.01.037"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20030422"},{"key":"e_1_2_5_25_2","first-page":"774","article-title":"A century\u2010old debate on protein aggregation and neurodegeneration enters the clinic","volume":"443","author":"Lansbury PT","year":"2006","journal-title":"Nat Cell Biol"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408277102"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja107552s"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2010.05.001"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.11.3881"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.23.8628"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-15-2-163"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0320865"},{"key":"e_1_2_5_33_2","doi-asserted-by":"crossref","first-page":"4173","DOI":"10.1016\/S0021-9258(19)39539-0","article-title":"Islet amyloid polypeptide\u2014a new beta\u2010cell secretory product related to islet amyloid deposits","volume":"265","author":"Nishi M","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_5_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00265542"},{"key":"e_1_2_5_35_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.48.2.241"},{"key":"e_1_2_5_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)90825-7"},{"key":"e_1_2_5_37_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-0405"},{"key":"e_1_2_5_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0531-5565(03)00004-4"},{"key":"e_1_2_5_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/368756a0"},{"key":"e_1_2_5_40_2","first-page":"151","article-title":"Islet amyloid, increased alpha\u2010cells, reduced beta\u2010cells and exocrine fibrosis\u2014quantitative changes in the pancreas in type\u20102 diabetes","volume":"9","author":"Clark A","year":"1988","journal-title":"Diabetes Res Clin Pract"},{"key":"e_1_2_5_41_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.1.102"},{"key":"e_1_2_5_42_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc0802893"},{"key":"e_1_2_5_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10328"},{"key":"e_1_2_5_44_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909024107"},{"key":"e_1_2_5_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2008.08.052"},{"key":"e_1_2_5_46_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.01976.x"},{"key":"e_1_2_5_47_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf051985c"},{"key":"e_1_2_5_48_2","doi-asserted-by":"publisher","DOI":"10.3109\/13506129509031881"},{"key":"e_1_2_5_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi702518m"},{"key":"e_1_2_5_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2009.07307.x"},{"key":"e_1_2_5_51_2","doi-asserted-by":"publisher","DOI":"10.1021\/ol047480"},{"key":"e_1_2_5_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2005.11.029"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.2023","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.2023","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T21:39:44Z","timestamp":1696887584000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.2023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,31]]},"references-count":51,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1002\/pro.2023"],"URL":"https:\/\/doi.org\/10.1002\/pro.2023","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,31]]}}}