{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T07:14:30Z","timestamp":1696922070697},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,4,24]],"date-time":"2012-04-24T00:00:00Z","timestamp":1335225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Program of National Nature Science Foundation of China","award":["31071751"],"award-info":[{"award-number":["31071751"]}]},{"name":"Genetically Modified Organism Breeding Major Project","award":["2011zx08005-002"],"award-info":[{"award-number":["2011zx08005-002"]}]},{"name":"Ministry of Science and Technology","award":["2008AA10Z127"],"award-info":[{"award-number":["2008AA10Z127"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Cynanchum komarovii<\/jats:italic> Al Iljinski is a desert plant that has been used as analgesic, anthelminthic, and antidiarrheal, but also as herbal medicine to treat cholecystitis in people. In this work, an antifungal protein with sequence homology to chitinase was isolated from <jats:italic>C<\/jats:italic>. <jats:italic>komarovii<\/jats:italic> seeds and named CkChn134. The three\u2010dimensional structure prediction of CkChn134 indicated that the protein has a loop domain formed a thin cleft, which is able to bind molecules and substrates. The protein and CkTLP synergistically inhibited the fungal growth of <jats:italic>Verticillium dahliae<\/jats:italic>, <jats:italic>Fusarium oxysporum<\/jats:italic>, <jats:italic>Rhizoctonia solani<\/jats:italic>, <jats:italic>Botrytis cinerea<\/jats:italic>, and <jats:italic>Valsa mali in vitro<\/jats:italic>. The full\u2010length cDNA was cloned by RT\u2010PCR and RACE\u2010PCR according to the partial protein sequences obtained by nanoESI\u2010MS\/MS. The real\u2010time PCR showed that the transcription level of <jats:italic>CkChn<\/jats:italic>134 had a significant increase under the stress of ethylene, NaCl, low temperature, drought, and pathogen infection, which indicates that CkChn134 may play an important role in response to abiotic and biotic stresses. The CkChn134 protein was located in the extracellular space\/cell wall by CkChn134::GFP fusion protein in transgenic Arabidopsis. Furthermore, overexpression of CkChn134 significantly enhanced the resistance of transgenic Arabidopsis against <jats:italic>V<\/jats:italic>. <jats:italic>dahliae<\/jats:italic>. Interestingly, the coexpression of CkChn134 and CkTLP showed substantially greater protection against the fungal pathogen <jats:italic>V. dahliae<\/jats:italic> than either transgene alone. The results suggest that the CkChn134 is a good candidate protein or gene, and it had a potential synergistic effect with CkTLP for contributing to the development of disease\u2010resistant crops.<\/jats:p>","DOI":"10.1002\/pro.2073","type":"journal-article","created":{"date-parts":[[2012,4,3]],"date-time":"2012-04-03T14:53:55Z","timestamp":1333464835000},"page":"865-875","source":"Crossref","is-referenced-by-count":3,"title":["Purification, characterization of a CkChn134 protein from <i>Cynanchum komarovii<\/i> seeds and synergistic effect with CkTLP against <i>Verticillium dahliae<\/i>"],"prefix":"10.1002","volume":"21","author":[{"given":"Qinghua","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxia","family":"Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sihong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowen","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,4,24]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(98)80058-X"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/07388550601168223"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.3923\/ajb.2011.29.37"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.254.5035.1194"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.2001.14.7.832"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.88.3.936"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0894-807"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1995.08010097.x"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00023946"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02981742"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1024276127001"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erg110"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016930"},{"key":"e_1_2_5_15_2","first-page":"12","article-title":"Breeding of a new germplasm cotton resistance to verticillium wilt","volume":"1","author":"Xiao S","year":"2007","journal-title":"China Cotton"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1107\/S1744309108007823"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2009.01.007"},{"key":"e_1_2_5_18_2","doi-asserted-by":"crossref","first-page":"19944","DOI":"10.1016\/S0021-9258(19)88648-9","article-title":"The complete amino acid sequence of yam (Dioscorea japonica) chitinase. 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