{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T17:04:00Z","timestamp":1788627840964,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,9,19]],"date-time":"2012-09-19T00:00:00Z","timestamp":1348012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Institutes of Health Center for Protein Structure and Function","award":["NCRR COBRE 1 P20RR15569 and INBRE P20RR16460; Grant sponsors: AR Biosciences Institute and Japan Society for the Promotion of Science and Kagawa University Project Research Fund 2005-2006"],"award-info":[{"award-number":["NCRR COBRE 1 P20RR15569 and INBRE P20RR16460; Grant sponsors: AR Biosciences Institute and Japan Society for the Promotion of Science and Kagawa University Project Research Fund 2005-2006"]}]},{"name":"MEXT, Japan","award":["19390032; Grant sponsors: E.I. DuPont de Nemours & Co., The Dow Chemical Company and Northwestern University"],"award-info":[{"award-number":["19390032; Grant sponsors: E.I. DuPont de Nemours & Co., The Dow Chemical Company and Northwestern University"]}]},{"name":"U.S. DOE","award":["DE-AC02-06CH11357"],"award-info":[{"award-number":["DE-AC02-06CH11357"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Clostridium histolyticum<\/jats:italic>\n                    collagenase causes extensive degradation of collagen in connective tissue that results in gas gangrene. The C\u2010terminal collagen\u2010binding domain (CBD) of these enzymes is the minimal segment required to bind to a collagen fibril. CBD binds unidirectionally to the undertwisted C\u2010terminus of triple helical collagen. Here, we examine whether CBD could also target undertwisted regions even in the middle of the triple helix. Collageneous peptides with an additional undertwisted region were synthesized by introducing a Gly \u2192 Ala substitution [(POG)\n                    <jats:italic>\n                      <jats:sub>x<\/jats:sub>\n                    <\/jats:italic>\n                    POA(POG)\n                    <jats:italic>\n                      <jats:sub>y<\/jats:sub>\n                    <\/jats:italic>\n                    ]\n                    <jats:sub>3<\/jats:sub>\n                    , where\n                    <jats:italic>x<\/jats:italic>\n                    +\n                    <jats:italic>y<\/jats:italic>\n                    = 9 and\n                    <jats:italic>x<\/jats:italic>\n                    &gt; 3).\n                    <jats:sup>1<\/jats:sup>\n                    H\u2013\n                    <jats:sup>15<\/jats:sup>\n                    N heteronuclear single quantum coherence nuclear magnetic resonance (HSQC NMR) titration studies with\n                    <jats:sup>15<\/jats:sup>\n                    N\u2010labeled CBD demonstrated that the minicollagen binds to a 10 \u00c5 wide 25 \u00c5 long cleft. Six collagenous peptides each labeled with a nitroxide radical were then titrated with\n                    <jats:sup>15<\/jats:sup>\n                    N\u2010labeled CBD. CBD binds to either the Gly \u2192 Ala substitution site or to the C\u2010terminus of each minicollagen. Small\u2010angle X\u2010ray scattering measurements revealed that CBD prefers to bind the Gly \u2192 Ala site to the C\u2010terminus. The HSQC NMR spectra of\n                    <jats:sup>15<\/jats:sup>\n                    N\u2010labeled minicollagen and minicollagen with undertwisted regions were unaffected by the titration of unlabeled CBD. The results imply that CBD binds to the undertwisted region of the minicollagen but does not actively unwind the triple helix.\n                  <\/jats:p>","DOI":"10.1002\/pro.2145","type":"journal-article","created":{"date-parts":[[2012,8,15]],"date-time":"2012-08-15T13:43:08Z","timestamp":1345038188000},"page":"1554-1565","source":"Crossref","is-referenced-by-count":27,"title":["Bacterial collagen\u2010binding domain targets undertwisted regions of collagen"],"prefix":"10.1002","volume":"21","author":[{"given":"Sagaya Theresa Leena","family":"Philominathan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaki","family":"Koide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Matsushita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Sakon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,19]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"2579","article-title":"Injectable collagenase clostridium histolyticum for Dupuytren's contracture","volume":"361","author":"Holzer LA","year":"2009","journal-title":"N Engl J Med"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.6.3643"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003450200"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.7018"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27379"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-011-9485-1"},{"key":"e_1_2_6_8_2","first-page":"e392","article-title":"Prevention of chemotherapy\u2010induced osteoporosis by cyclophosphamide with a long\u2010acting form of parathyroid hormone","volume":"34","author":"Ponnapakkam T","year":"2011","journal-title":"J Endocrinol Invest"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg172"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2009.07078.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-011-0309-3"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M807684200"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.32602"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi952270d"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00080a007"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0710588105"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2010.02.003"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008200109016842"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21687"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9021473"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(02)00421-7"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5193(05)80157-2"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1080\/03008200802151698"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7695699"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1004(1997)9:4<300::AID-HUMU2>3.0.CO;2-9"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12104-008-9102-z"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00211a043"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2011.06.017"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.018077"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2007.06.009"},{"key":"e_1_2_6_31_2","author":"Proteau A","year":"2010","journal-title":"Curr Protoc Protein Sci"},{"key":"e_1_2_6_32_2","first-page":"58","volume":"52","author":"Weigand S","year":"2009","journal-title":"Advances in X\u2010ray Analysis. 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