{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:28:37Z","timestamp":1758925717988,"version":"3.37.3"},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,10,26]],"date-time":"2012-10-26T00:00:00Z","timestamp":1351209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["EY012347","RR11973"],"award-info":[{"award-number":["EY012347","RR11973"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Flagellar calcium binding proteins are expressed in a variety of trypanosomes and are potential drug targets for Chagas disease and African sleeping sickness. The flagellar calcium binding protein calflagin of <jats:italic>Trypanosoma brucei<\/jats:italic> (called Tb24) is a myristoylated and palmitoylated EF\u2010hand protein that is targeted to the inner leaflet of the flagellar membrane. The Tb24 protein may also interact with proteins on the membrane surface that may be different from those bound to flagellar calcium binding proteins (FCaBPs) in <jats:italic>T. cruzi<\/jats:italic>. We report here the NMR structure of Tb24 that contains four EF\u2010hand motifs bundled in a compact arrangement, similar to the overall fold of <jats:italic>T. cruzi<\/jats:italic> FCaBP (RMSD = 1.0 \u00c5). A cluster of basic residues (K22, K25, K31, R36, and R38) located on a surface near the N\u2010terminal myristoyl group may be important for membrane binding. Non\u2010conserved residues on the surface of a hydrophobic groove formed by EF2 (P91, Q95, D103, and V108) and EF4 (C194, T198, K199, Q202, and V203) may serve as a target protein binding site and could have implications for membrane target recognition.<\/jats:p>","DOI":"10.1002\/pro.2167","type":"journal-article","created":{"date-parts":[[2012,9,26]],"date-time":"2012-09-26T03:23:49Z","timestamp":1348629829000},"page":"1942-1947","source":"Crossref","is-referenced-by-count":7,"title":["NMR structure of the calflagin Tb24 flagellar calcium binding protein of <i>Trypanosoma brucei<\/i>"],"prefix":"10.1002","volume":"21","author":[{"given":"Xianzhong","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl L.","family":"Olson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Engman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James B.","family":"Ames","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,10,26]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"crossref","first-page":"18627","DOI":"10.1016\/S0021-9258(18)51512-X","article-title":"A novel flagellar Ca2+\u2010binding protein in trypanosomes","volume":"264","author":"Engman DM","year":"1989","journal-title":"J Biol 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NMR Assign.","article-title":"1H, 15N, and 13C chemical shift assignments of the calflagin Tb24 flagellar calcium binding protein of Trypanosoma brucei","author":"Xu X","key":"e_1_2_7_21_2"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)89042-8"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00471a022"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00076a099"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00197809"},{"key":"e_1_2_7_26_2","first-page":"37461","article-title":"Structural analysis of Mg2+ and Ca2+ binding to CaBP1, a neuron\u2010specific regulator of calcium channels","volume":"280","author":"Schwieters CD","year":"2003","journal-title":"J Biol Chem"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008392405740"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/2.1.27"}],"container-title":["Protein 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