{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:39:28Z","timestamp":1791149968967,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,3,18]],"date-time":"2013-03-18T00:00:00Z","timestamp":1363564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["R56 R56AI090085"],"award-info":[{"award-number":["R56 R56AI090085"]}]},{"name":"NIH","award":["R01AI090085"],"award-info":[{"award-number":["R01AI090085"]}]},{"name":"NIH","award":["SR37AI022021"],"award-info":[{"award-number":["SR37AI022021"]}]},{"name":"NIH","award":["NIGMS P41 GM103310"],"award-info":[{"award-number":["NIGMS P41 GM103310"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have visualized by cryo\u2010electron microscopy (cryo\u2010EM) the complex of the anthrax protective antigen (PA) translocon and the N\u2010terminal domain of anthrax lethal factor (LF<jats:sub>N<\/jats:sub>) inserted into a nanodisc model lipid bilayer. We have determined the structure of this complex at a nominal resolution of 16 \u00c5 by single\u2010particle analysis and three\u2010dimensional reconstruction. Consistent with our previous analysis of negatively stained unliganded PA, the translocon comprises a globular structure (cap) separated from the nanodisc bilayer by a narrow stalk that terminates in a transmembrane channel (incompletely distinguished in this reconstruction). The globular cap is larger than the unliganded PA pore, probably due to distortions introduced in the previous negatively stained structures. The cap exhibits larger, more distinct radial protrusions, previously identified with PA domain three, fitted by elements of the NMFF PA prepore crystal structure. The presence of LF<jats:sub>N<\/jats:sub>, though not distinguished due to the seven\u2010fold averaging used in the reconstruction, contributes to the distinct protrusions on the cap rim volume distal to the membrane. Furthermore, the lumen of the cap region is less resolved than the unliganded negatively stained PA, due to the low contrast obtained in our images of this specimen. Presence of the LF<jats:sub>N<\/jats:sub> extended helix and N terminal unstructured regions may also contribute to this additional internal density within the interior of the cap. Initial NMFF fitting of the cryoEM\u2010defined PA pore cap region positions the Phe clamp region of the PA pore translocon directly above an internal vestibule, consistent with its role in toxin translocation.<\/jats:p>","DOI":"10.1002\/pro.2241","type":"journal-article","created":{"date-parts":[[2013,3,14]],"date-time":"2013-03-14T20:40:24Z","timestamp":1363293624000},"page":"586-594","source":"Crossref","is-referenced-by-count":31,"title":["Three dimensional structure of the anthrax toxin translocon\u2013lethal factor complex by cryo\u2010electron microscopy"],"prefix":"10.1002","volume":"22","author":[{"given":"E. P.","family":"Gogol","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Akkaladevi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Szerszen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Mukherjee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Chollet\u2010Hinton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Katayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. L.","family":"Pentelute","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. J.","family":"Collier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. T.","family":"Fisher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,3,18]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.2052"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.199"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1923"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1442"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000100107"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1113380"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.2231"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.2000.4314"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2003.09.033"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(10)83015-0"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(09)64011-8"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1018965108"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi1017446"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi990792d"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M401292200"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2005.03.010"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2009.01.002"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultramic.2005.02.004"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-842-3_9"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1996.0030"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.5133"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.01.048"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20906"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.03154503"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2011.09.022"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.2241","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.2241","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.2241","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T06:42:34Z","timestamp":1694760154000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.2241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3,18]]},"references-count":26,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,5]]}},"alternative-id":["10.1002\/pro.2241"],"URL":"https:\/\/doi.org\/10.1002\/pro.2241","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,18]]}}}