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To understand the influence of the intracellular environment on stability, one must know the effect of the cytoplasm on these rates. We probed exchange rates in buffer and in <jats:italic>Escherichia coli<\/jats:italic> lysates for the dynamic loop in the small globular protein chymotrypsin inhibitor 2 using a modified form of the nuclear magnetic resonance experiment, SOLEXSY. No significant changes were observed, even in 100 g dry weight L<jats:sup>\u22121<\/jats:sup> lysate. Our results suggest that intrinsic rates from studies conducted in buffers are applicable to studies conducted under cellular conditions.<\/jats:p>","DOI":"10.1002\/pro.2318","type":"journal-article","created":{"date-parts":[[2013,7,31]],"date-time":"2013-07-31T14:26:42Z","timestamp":1375280802000},"page":"1313-1319","source":"Crossref","is-referenced-by-count":19,"title":["Amide proton exchange of a dynamic loop in cell extracts"],"prefix":"10.1002","volume":"22","author":[{"given":"Austin E.","family":"Smith","sequence":"first","affiliation":[{"name":"Department of Chemistry University of North Carolina Chapel Hill North Carolina 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohona","family":"Sarkar","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of North Carolina Chapel Hill North Carolina 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory B.","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics University of North Carolina Chapel Hill North Carolina 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary J.","family":"Pielak","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of North Carolina Chapel Hill North Carolina 27599"},{"name":"Department of Biochemistry and Biophysics University of North Carolina Chapel Hill North Carolina 27599"},{"name":"Lineberger Comprehensive Cancer Center University of North Carolina Chapel Hill North Carolina 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,8,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1000694"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(91)90499-V"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.37.032807.125817"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2010.01.008"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(54)90241-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(57)90478-2"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560060517"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0033583500005217"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi900526z"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(09)66001-8"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3233(08)60129-1"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340200103"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1088"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340170110"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340170111"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00752a003"},{"key":"e_1_2_7_18_1","volume-title":"Protein and peptide structure and interactions studied by hydrogen exchange and NMR","author":"Zhang Y\u2010Z","year":"1995"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi300909q"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja8005995"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja200067p"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja305300m"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00046a016"},{"key":"e_1_2_7_24_1","unstructured":"The PyMOLMolecular Graphics System Version 1.3. 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