{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T17:03:04Z","timestamp":1792602184006,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2009,8,21]],"date-time":"2009-08-21T00:00:00Z","timestamp":1250812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Protein aggregation is a common feature of late onset neurodegenerative disorders, including Alzheimer's disease. In Alzheimer's disease, misassembly of the A\u03b2 peptide is genetically linked to proteotoxicity associated with disease etiology. A reduction in A\u03b2 proteotoxicity is accomplished, in part, by the previously reported A\u03b2 disaggregation and proteolysis activities\u2013under partial control of heat shock factor 1, a transcription factor regulating proteostasis in the cytosol and negatively regulated by insulin growth factor signaling. Herein, we report an improved <jats:italic>in vitro<\/jats:italic> assay to quantify recombinant fibrillar A\u03b2 disaggregation kinetics accomplished by the exogenous application of <jats:italic>C.<\/jats:italic><jats:italic>elegans<\/jats:italic> extracts. With this assay we demonstrate that the A\u03b2 disaggregation and proteolysis activities of <jats:italic>C.<\/jats:italic><jats:italic>elegans<\/jats:italic> are separable. The disaggregation activity found in <jats:italic>C.<\/jats:italic><jats:italic>elegans<\/jats:italic> preparations is more heat resistant than the proteolytic activity. A\u03b2 disaggregation in the absence of proteolysis was found to be a reversible process. Future discovery of the molecular basis of the disaggregation and proteolysis activities offers the promise of delaying the age\u2010onset proteotoxicity that leads to neurodegeneration in a spectrum of maladies.<\/jats:p>","DOI":"10.1002\/pro.234","type":"journal-article","created":{"date-parts":[[2009,8,21]],"date-time":"2009-08-21T18:25:04Z","timestamp":1250879104000},"page":"2231-2241","source":"Crossref","is-referenced-by-count":28,"title":["A kinetic assessment of the <i>C. elegans<\/i> amyloid disaggregation activity enables uncoupling of disassembly and 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