{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:02:25Z","timestamp":1790420545057,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2009,8,31]],"date-time":"2009-08-31T00:00:00Z","timestamp":1251676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Domain 4 of the anthrax protective antigen (PA) plays a key role in cellular receptor recognition as well as in pH\u2010dependent pore formation. We present here the 1.95 \u00c5 crystal structure of domain 4, which adopts a fold that is identical to that observed in the full\u2010length protein. We have also investigated the structural properties of the isolated domain 4 as a function of pH, as well as the pH\u2010dependence on binding to the von Willebrand factor A domain of capillary morphogenesis protein 2 (CMG2). Our results provide evidence that the isolated domain 4 maintains structure and interactions with CMG2 at pH 5, a pH that is known to cause release of the receptor on conversion of the heptameric prepore (PA<jats:sub>63<\/jats:sub>)<jats:sub>7<\/jats:sub>to a membrane\u2010spanning pore. Our results suggest that receptor release is not driven solely by a pH\u2010induced unfolding of domain 4.<\/jats:p>","DOI":"10.1002\/pro.238","type":"journal-article","created":{"date-parts":[[2009,8,31]],"date-time":"2009-08-31T20:25:35Z","timestamp":1251750335000},"page":"2277-2286","source":"Crossref","is-referenced-by-count":24,"title":["Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH"],"prefix":"10.1002","volume":"18","author":[{"given":"Alexander S.","family":"Williams","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott","family":"Lovell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asokan","family":"Anbanandam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahif","family":"El\u2010Chami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James G.","family":"Bann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,10,19]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/n35101999"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M401292200"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0431098100"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.15.2755"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.75.103004.142728"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200312072"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1113380"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.11.030"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405405101"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0505865102"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401506101"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307900200"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02763"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000329"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0119518"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi972657b"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.26"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/385833a0"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M609869200"},{"key":"e_1_2_6_21_2","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/S1046-5928(02)00612-5","article-title":"Expression and purification of the Bacillus anthracis protective antigen","volume":"27","author":"Krishnanchettiar JSS","year":"2002","journal-title":"Protein Expr Purif"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00342a032"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00421a017"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00336a004"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00375a025"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3233(08)60520-3"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/16.6.566"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00923-9"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(84)71582-8"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00447a048"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi048614u"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi990792d"},{"key":"e_1_2_6_33_2","first-page":"1","volume-title":"Current Protocols in Protein Science","author":"Bernard A","year":"1995"},{"key":"e_1_2_6_34_2","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1093\/oso\/9780199636198.003.0010","volume-title":"Protein Structure: A Practical Approach","author":"Pace CN","year":"1997"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00065a035"},{"key":"e_1_2_6_36_2","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1093\/oso\/9780199636198.003.0012","volume-title":"Protein Structure: A Practical Approach","author":"Pace CN","year":"1997"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00197809"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)76066-X"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444905036693"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889897006766"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444996012255"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.10286"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)77027-7"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.238","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.238","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.238","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T05:49:20Z","timestamp":1710568160000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.238"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10,19]]},"references-count":43,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2009,11]]}},"alternative-id":["10.1002\/pro.238"],"URL":"https:\/\/doi.org\/10.1002\/pro.238","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,10,19]]}}}