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Like other bacterial diderms, it must transport these biochemicals from the extracellular milieu across two membranes, ultimately to the <jats:italic>B. burgdorferi<\/jats:italic> cytoplasm. In the current study, we established that a gene cluster comprising genes <jats:italic>bb0215<\/jats:italic> through <jats:italic>bb0218<\/jats:italic> is cotranscribed and is therefore an operon. Sequence analysis of these proteins suggested that they are the components of an ABC\u2010type transporter responsible for translocating phosphate anions from the <jats:italic>B. burgdorferi<\/jats:italic> periplasm to the cytoplasm. Biophysical experiments established that the putative ligand\u2010binding protein of this system, BbPstS (BB0215), binds to phosphate in solution. We determined the high\u2010resolution (1.3 \u00c5) crystal structure of the protein in the absence of phosphate, revealing that the protein's fold is similar to other phosphate\u2010binding proteins, and residues that are implicated in phosphate binding in other such proteins are conserved in BbPstS. Taken together, the gene products of <jats:italic>bb0215\u20100218<\/jats:italic> function as a phosphate transporter for <jats:italic>B. burgdorferi<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/pro.2406","type":"journal-article","created":{"date-parts":[[2013,12,9]],"date-time":"2013-12-09T02:08:39Z","timestamp":1386554919000},"page":"200-212","source":"Crossref","is-referenced-by-count":16,"title":["Sequence, biophysical, and structural analyses of the PstS lipoprotein (BB0215) from <b><i>Borrelia burgdorferi<\/i><\/b> reveal a likely binding component of an ABC\u2010type phosphate transporter"],"prefix":"10.1002","volume":"23","author":[{"given":"Chad A.","family":"Brautigam","sequence":"first","affiliation":[{"name":"Department of Biophysics The University of Texas Southwestern Medical Center Dallas Texas 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiming","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Department of Microbiology The University of Texas Southwestern Medical Center Dallas Texas 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjit K.","family":"Deka","sequence":"additional","affiliation":[{"name":"Department of Microbiology The University of Texas Southwestern Medical Center Dallas Texas 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael V.","family":"Norgard","sequence":"additional","affiliation":[{"name":"Department of Microbiology The University of Texas Southwestern Medical Center Dallas Texas 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