{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T21:11:10Z","timestamp":1785791470446,"version":"3.56.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,11,4]],"date-time":"2014-11-04T00:00:00Z","timestamp":1415059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institute for Health Research","doi-asserted-by":"crossref","award":["MOP-89787"],"award-info":[{"award-number":["MOP-89787"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bacteria have evolved cellular control mechanisms to ensure proper length specification for surface\u2010bound polysaccharides. Members of the Polysaccharide Copolymerase (PCP) family are central to this process. PCP\u20101 family members are anchored to the inner membrane through two transmembrane helices and contain a large periplasm\u2010exposed domain. PCPs are known to form homooligomers but their exact stoichiometry is controversial in view of conflicting structural and biochemical data. Several prior investigations addressing this question indicated a nonameric, hexameric, or tetrameric organization of several PCP\u20101 family members. In this work, we gathered additional evidence that <jats:italic>E.coli<\/jats:italic> WzzB and WzzE PCPs form octameric homo\u2010oligomeric complexes. Detergent\u2010solubilized PCPs were purified to homogeneity and subjected to blue native gel analysis, which indicated the presence of a predominant high\u2010molecular product of over 500 kDa in mass. Molecular mass of WzzE and WzzB\u2010detergent oligomers was estimated to be 550 kDA by size\u2010exclusion coupled to multiangle laser light scattering (SEC\u2010MALLS). Oligomeric organization of purified WzzB and WzzE was further investigated by negative stain electron microscopy and by X\u2010ray crystallography, respectively. Analysis of EM\u2010derived molecular envelope of WzzB indicated that the full\u2010length protein is composed of eight protomers. Crystal structure of LDAO\u2010solubilized WzzE was solved to 6 \u00c5 resolutions and revealed its octameric subunit stoichiometry. In summary, we identified a possible biological unit utilized for the glycan chain length determination by two PCP\u20101 family members. This provides an important step toward further unraveling of the mechanistic basis of chain length control of the O\u2010antigen and the enterobacterial common antigen.<\/jats:p>","DOI":"10.1002\/pro.2586","type":"journal-article","created":{"date-parts":[[2014,10,11]],"date-time":"2014-10-11T05:13:12Z","timestamp":1413004392000},"page":"58-69","source":"Crossref","is-referenced-by-count":24,"title":["Quaternary structure of <scp>W<\/scp>zzB and <scp>W<\/scp>zzE polysaccharide copolymerases"],"prefix":"10.1002","volume":"24","author":[{"given":"Sergei","family":"Kalynych","sequence":"first","affiliation":[{"name":"Department of Biochemistry McGill University Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maia","family":"Cherney","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Saskatchewan Saskatoon Saskatchewan Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihnea","family":"Bostina","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Otago Dunedin New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Rouiller","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology McGill University Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslaw","family":"Cygler","sequence":"additional","affiliation":[{"name":"Department of Biochemistry McGill University Montreal Quebec Canada"},{"name":"Department of Biochemistry University of Saskatchewan Saskatoon Saskatchewan 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