{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:00:16Z","timestamp":1787162416385,"version":"build-2736575974"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,12,3]],"date-time":"2014-12-03T00:00:00Z","timestamp":1417564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Health and Medical Research Council Fellowship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The oral pathogen\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    is a keystone pathogen in the development of chronic periodontitis. Gingipains, the principle virulence factors of\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    are multidomain, cell\u2010surface proteins containing a cysteine protease domain. The lysine specific gingipain, Kgp, is a critical virulence factor of\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    . We have determined the X\u2010ray crystal structure of the lysine\u2010specific protease domain of Kgp to 1.6 \u00c5 resolution. The structure provides insights into the mechanism of substrate specificity and catalysis.\n                  <\/jats:p>","DOI":"10.1002\/pro.2589","type":"journal-article","created":{"date-parts":[[2014,10,18]],"date-time":"2014-10-18T09:03:11Z","timestamp":1413622991000},"page":"162-166","source":"Crossref","is-referenced-by-count":20,"title":["Structure of the lysine specific protease\n                    <scp>K<\/scp>\n                    gp from\n                    <scp>\n                      <i>P<\/i>\n                    <\/scp>\n                    <i>orphyromonas gingivalis<\/i>\n                    , a target for improved oral health"],"prefix":"10.1002","volume":"24","author":[{"given":"Michael A.","family":"Gorman","sequence":"first","affiliation":[{"name":"ACRF Rational Drug Discovery Centre St. Vincent's Institute of Medical Research Fitzroy Victoria 3065 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine A.","family":"Seers","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre, Melbourne Dental School Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne Melbourne Victoria 3010 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belinda J.","family":"Michell","sequence":"additional","affiliation":[{"name":"ACRF Rational Drug Discovery Centre St. Vincent's Institute of Medical Research Fitzroy Victoria 3065 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne C.","family":"Feil","sequence":"additional","affiliation":[{"name":"ACRF Rational Drug Discovery Centre St. Vincent's Institute of Medical Research Fitzroy Victoria 3065 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. Laila","family":"Huq","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre, Melbourne Dental School Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne Melbourne Victoria 3010 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith J.","family":"Cross","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre, Melbourne Dental School Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne Melbourne Victoria 3010 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric C.","family":"Reynolds","sequence":"additional","affiliation":[{"name":"Oral Health Cooperative Research Centre, Melbourne Dental School Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne Melbourne Victoria 3010 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael W.","family":"Parker","sequence":"additional","affiliation":[{"name":"ACRF Rational Drug Discovery Centre St. Vincent's Institute of Medical Research Fitzroy Victoria 3065 Australia"},{"name":"Department of Biochemistry and Molecular Biology Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne Melbourne Victoria 3010 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01627-06"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02004-06"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/019943-0"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01038-08"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2873"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065447"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1999.tb02282.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471140864.ps2120s49"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.20.5453"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.444927"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444999010495"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0909049502015170"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909047374"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444905036693"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444902016657"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.360221211"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.2589","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.2589","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.2589","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T20:36:43Z","timestamp":1696365403000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.2589"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,3]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1002\/pro.2589"],"URL":"https:\/\/doi.org\/10.1002\/pro.2589","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,3]]}}}