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The sequences were compared to human apoE4 to determine conserved and non\u2010conserved sequences of amino acids. ApoE4 is the major risk factor for the development of late onset Alzheimer's disease while apoE3, which differs from apoE4 by a single amino acid change at position 112, poses little or no risk for the development of this disease. Thus, the two proteins appear to be structurally and functionally different. Seven highly conserved regions, representing approximately 47 amino acids (of 299) have been found. These regions are distributed throughout the protein and reflect ligand binding sites as well as regions proposed to be involved in the propagation of the cysteine\u2013arginine change at position 112 to distant regions of the protein in the N\u2010 and C\u2010terminal domains. Highly non\u2010conserved regions are at the N\u2010 and C\u2010terminal ends of the apoE protein.<\/jats:p>","DOI":"10.1002\/pro.2597","type":"journal-article","created":{"date-parts":[[2014,11,6]],"date-time":"2014-11-06T21:54:17Z","timestamp":1415310857000},"page":"138-144","source":"Crossref","is-referenced-by-count":25,"title":["Apo<scp>E<\/scp>: The role of conserved residues in defining function"],"prefix":"10.1002","volume":"24","author":[{"given":"Carl","family":"Frieden","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics Washington University School of Medicine St. Louis Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047760"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909606106"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-4580(99)00053-6"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52510-8"},{"key":"e_1_2_5_6_1","first-page":"4171","article-title":"Human apolipoprotein E","volume":"257","author":"Rall SC","year":"1982","journal-title":"The complete amino acid sequence. 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