{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T01:43:36Z","timestamp":1776822216686,"version":"3.51.2"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,3,10]],"date-time":"2015-03-10T00:00:00Z","timestamp":1425945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Intramural Research Program of the NIH, NIAID"},{"name":"NIH Oxford-Cambridge Scholars Program. X-ray data were collected at the Southeast Regional Collaborative Access Team (SER-CAT) 22-ID and 22-BM beam lines at the Advanced Photon Source"},{"name":"U. S. Department of Energy, Office of Science, Office of Basic Energy Sciences","award":["contract W-31-109-Eng-38"],"award-info":[{"award-number":["contract W-31-109-Eng-38"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Coenzyme F<jats:sub>420<\/jats:sub> is a deazaflavin hydride carrier with a lower reduction potential than most flavins. In <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (<jats:italic>Mtb<\/jats:italic>), F<jats:sub>420<\/jats:sub> plays an important role in activating PA\u2010824, an antituberculosis drug currently used in clinical trials. Although F<jats:sub>420<\/jats:sub> is important to <jats:italic>Mtb<\/jats:italic> redox metabolism, little is known about the enzymes that bind F<jats:sub>420<\/jats:sub> and the reactions that they catalyze. We have identified a novel F<jats:sub>420<\/jats:sub>\u2010binding protein, Rv1155, which is annotated in the <jats:italic>Mtb<\/jats:italic> genome sequence as a putative flavin mononucleotide (FMN)\u2010binding protein. Using biophysical techniques, we have demonstrated that instead of binding FMN or other flavins, Rv1155 binds coenzyme F<jats:sub>420<\/jats:sub>. The crystal structure of the complex of Rv1155 and F<jats:sub>420<\/jats:sub> reveals one F<jats:sub>420<\/jats:sub> molecule bound to each monomer of the Rv1155 dimer. Structural, biophysical, and bioinformatic analyses of the Rv1155\u2013F<jats:sub>420<\/jats:sub> complex provide clues about its role in the bacterium.<\/jats:p>","DOI":"10.1002\/pro.2645","type":"journal-article","created":{"date-parts":[[2015,1,31]],"date-time":"2015-01-31T07:29:32Z","timestamp":1422689372000},"page":"729-740","source":"Crossref","is-referenced-by-count":20,"title":["Molecular insights into the binding of coenzyme <scp>F<sub>420<\/sub><\/scp> to the conserved protein <scp>R<\/scp>v1155 from <scp><i>M<\/i><\/scp><i>ycobacterium tuberculosis<\/i>"],"prefix":"10.1002","volume":"24","author":[{"given":"Ellene H.","family":"Mashalidis","sequence":"first","affiliation":[{"name":"Tuberculosis Research Section, National Institute of Allergy and Infectious Diseases Bethesda Maryland 20892"},{"name":"Department of Chemistry University of Cambridge Cambridge CB2 1EW United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Apostolos G.","family":"Gittis","sequence":"additional","affiliation":[{"name":"Structural Biology Section Research Technologies Branch, National Institute of Allergy and Infectious Diseases Rockville Maryland 20852"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aurelie","family":"Tomczak","sequence":"additional","affiliation":[{"name":"Molecular Signaling Section, Laboratory of Molecular Immunology, National Institute of Allergy and Infectious Diseases Bethesda Maryland 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"Abell","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Cambridge Cambridge CB2 1EW United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Clifton E.","family":"Barry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David N.","family":"Garboczi","sequence":"additional","affiliation":[{"name":"Structural Biology Section Research Technologies Branch, National Institute of Allergy and Infectious Diseases Rockville Maryland 20852"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,3,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09657"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60727-2"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612043383214"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.112.1.527-531.1972"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002030100290"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/35016103"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00106-09"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(60)91396-2"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(64)90477-2"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar00127a004"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781444305364"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1065"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00425-10"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0508392103"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801854200"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.10.2861-2866.1996"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027643"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.11.069"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444905026673"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.130"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2011.11.001"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07356.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00316a008"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-1280(02)00719-4"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl282"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4734"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.0356203"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq366"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1107\/S1744309106025012"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-132"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg034"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.8.4285"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)01019-6"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.23.6561"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi901104d"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2004.02.010"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.041289805"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015803"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.11.5750-5755.2002"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.9.2420-2428.2002"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2007.01.014"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf000689r"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0378207"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.406"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904023509"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1094"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1701"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4315"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-255"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2010.09.008"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.2645","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.2645","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.2645","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pro.2645","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.2645","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T10:02:48Z","timestamp":1692439368000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.2645"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,10]]},"references-count":52,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1002\/pro.2645"],"URL":"https:\/\/doi.org\/10.1002\/pro.2645","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,10]]}}}