{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:07:42Z","timestamp":1792606062212,"version":"4.5.0"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,8,23]],"date-time":"2016-08-23T00:00:00Z","timestamp":1471910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"United States National Institute of Health","award":["GM078114, to D.P.R."],"award-info":[{"award-number":["GM078114, to D.P.R."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Amyloid deposition underlies a broad range of diseases including multiple neurodegenerative diseases, systemic amyloidosis and type\u20102 diabetes. Amyloid sensitive dyes, particularly thioflavin\u2010T, are widely used to detect <jats:italic>ex\u2010vivo<\/jats:italic> amyloid deposits, to monitor amyloid formation <jats:italic>in vitro<\/jats:italic> and to follow the kinetics of amyloid self\u2010assembly. We show that the dye SYPRO\u2010orange binds to amyloid fibrils formed by human amylin, the polypeptide responsible for islet amyloid formation in type\u20102 diabetes. No fluorescence enhancement is observed in the presence of pre\u2010fibrillar species or in the presence of non\u2010amyloidogenic rat amylin. The kinetics of human amylin amyloid formation can be monitored by SYPRO\u2010orange fluorescence and match the time course determined with thioflavin\u2010T assays. Thus, SYPRO\u2010orange offers an alternative to thioflavin\u2010T assays of amylin amyloid formation. The implications for the interpretation of SYPRO\u2010orange\u2010based assays of protein stability and protein\u2010ligand interactions are discussed.<\/jats:p>","DOI":"10.1002\/pro.2992","type":"journal-article","created":{"date-parts":[[2016,8,2]],"date-time":"2016-08-02T18:01:34Z","timestamp":1470160894000},"page":"1834-1840","source":"Crossref","is-referenced-by-count":10,"title":["The dye SYPRO orange binds to amylin amyloid fibrils but not pre\u2010fibrillar intermediates"],"prefix":"10.1002","volume":"25","author":[{"given":"Amy G.","family":"Wong","sequence":"first","affiliation":[{"name":"Department of Chemistry Stony Brook University Stony Brook New York 11794\u20103400"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel P.","family":"Raleigh","sequence":"additional","affiliation":[{"name":"Department of Chemistry Stony Brook University Stony Brook New York 11794\u20103400"},{"name":"Graduate Program in Biochemistry and Structural Biology Stony Brook University Stony Brook New York 11794"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.11.3881"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.23.8628"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.75.101304.123901"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1104-1054"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1067484"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560020312"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2010.04.001"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.5b01107"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi1016217"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi702518m"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2009.07307.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.0320"},{"key":"e_1_2_7_14_1","article-title":"Merocyanine dye protein stains","author":"RP Haugland","year":"1996","journal-title":"US Patent WO"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0354-2_19"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja8049063"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.04.031"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12249-013-0026-2"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00042.2009"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2798269"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2012.11.003"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi3014278"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2129"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.13.5036"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(02)01377-3"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi401164k"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/ol047480"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.5b00309"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb500505m"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/ol101981b"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.2992","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.2992","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.2992","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T05:00:56Z","timestamp":1696482056000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.2992"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,23]]},"references-count":29,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1002\/pro.2992"],"URL":"https:\/\/doi.org\/10.1002\/pro.2992","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,23]]}}}