{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T07:05:49Z","timestamp":1773731149818,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,9,16]],"date-time":"2016-09-16T00:00:00Z","timestamp":1473984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM115921"],"award-info":[{"award-number":["GM115921"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Kijanimicin is an antitumor antibiotic isolated from <jats:italic>Actinomadura kijaniata<\/jats:italic>. It is composed of three distinct moieties: a pentacyclic core, a monosaccharide referred to as <jats:sc>d<\/jats:sc>\u2010kijanose, and a tetrasaccharide chain composed of <jats:sc>l<\/jats:sc>\u2010digitoxose units. <jats:sc>d<\/jats:sc>\u2010Kijanose is a highly unusual nitro\u2010containing tetradeoxysugar, which requires at least ten enzymes for its production. Here we describe a structural analysis of one of these enzymes, namely KijD1, which functions as a <jats:italic>C<\/jats:italic>\u20103\u2032\u2010methyltransferase using <jats:italic>S<\/jats:italic>\u2010adenosylmethionine as its cofactor. For this investigation, two ternary complexes of KijD1, determined in the presence of <jats:italic>S<\/jats:italic>\u2010adenosylhomocysteine (SAH) and dTDP or SAH and dTDP\u20103\u2010amino\u20102,3,6\u2010trideoxy\u20104\u2010keto\u20103\u2010methyl\u2010<jats:sc>d<\/jats:sc>\u2010glucose, were solved to 1.7 or 1.6\u00a0\u00c5 resolution, respectively. Unexpectedly, these structures, as well as additional biochemical analyses, demonstrated that the quaternary structure of KijD1 is a dimer. Indeed, this is in sharp contrast to that previously observed for the sugar <jats:italic>C<\/jats:italic>\u20103\u2032\u2010methyltransferase isolated from <jats:italic>Micromonospora chalcea<\/jats:italic>. By the judicious use of site\u2010directed mutagenesis, it was possible to convert the dimeric form of KijD1 into a monomeric version. The quaternary structure of KijD1 could not have been deduced based solely on bioinformatics approaches, and thus this investigation highlights the continuing need for experimental validation.<\/jats:p>","DOI":"10.1002\/pro.3034","type":"journal-article","created":{"date-parts":[[2016,9,6]],"date-time":"2016-09-06T09:13:04Z","timestamp":1473153184000},"page":"2282-2289","source":"Crossref","is-referenced-by-count":3,"title":["Structural studies on KijD1, a sugar <i>C<\/i>\u20103\u2032\u2010methyltransferase"],"prefix":"10.1002","volume":"25","author":[{"given":"Garrett T.","family":"Dow","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Wisconsin Madison WI 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James B.","family":"Thoden","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Wisconsin Madison WI 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hazel M.","family":"Holden","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Wisconsin Madison WI 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.34.1101"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/p19830001497"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.36.1078"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/np500757w"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0744854"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi100782b"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.2074"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(99)00037-8"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(88)90606-7"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.33.668"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505730200"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi400407x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444996012255"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1351"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.3034","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.3034","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.3034","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T15:35:19Z","timestamp":1694273719000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.3034"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,16]]},"references-count":17,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1002\/pro.3034"],"URL":"https:\/\/doi.org\/10.1002\/pro.3034","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,16]]}}}