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Central to the pathogenesis of African trypanosomes is their transmission by the arthropod vector, <jats:italic>Glossina spp<\/jats:italic>. (tsetse fly). Intriguingly, the efficiency of parasite transmission through the vector is reduced following depletion of <jats:italic>Trypanosoma brucei<\/jats:italic> Procyclic\u2010Specific Surface Antigen\u20102 (<jats:italic>Tb<\/jats:italic>PSSA\u20102). To investigate the underlying molecular mechanism of <jats:italic>Tb<\/jats:italic>PSSA\u20102, we determined the crystal structures of its ectodomain and that of its homolog <jats:italic>T. congolense<\/jats:italic> Insect Stage Antigen (<jats:italic>Tc<\/jats:italic>ISA) to resolutions of 1.65 \u00c5 and 2.45 \u00c5, respectively using single wavelength anomalous dispersion. Both proteins adopt a novel bilobed architecture with the individual lobes displaying rotational flexibility around the central tether that suggest a potential mechanism for coordinating a binding partner. In support of this hypothesis, electron density consistent with a bound peptide was observed in the inter\u2010lob cleft of a <jats:italic>Tc<\/jats:italic>ISA monomer. These first reported structures of insect stage transmembrane proteins expressed by African trypanosomes provide potentially valuable insight into the interface between parasite and tsetse vector.<\/jats:p>","DOI":"10.1002\/pro.3053","type":"journal-article","created":{"date-parts":[[2016,9,27]],"date-time":"2016-09-27T06:35:20Z","timestamp":1474958120000},"page":"2297-2302","source":"Crossref","is-referenced-by-count":1,"title":["Structural characterization reveals a novel bilobed architecture for the ectodomains of insect stage expressed <i>Trypanosoma brucei<\/i> PSSA\u20102 and <i>Trypanosoma congolense<\/i> ISA"],"prefix":"10.1002","volume":"25","author":[{"given":"Raghavendran","family":"Ramaswamy","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Microbiology University of Victoria Victoria BC Canada V8W 3P6"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Goomeshi 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