{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T07:42:35Z","timestamp":1762674155419},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,2,9]],"date-time":"2017-02-09T00:00:00Z","timestamp":1486598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM115921"],"award-info":[{"award-number":["GM115921"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Campylobacter jejuni<\/jats:italic> is a Gram\u2010negative bacterium that represents a leading cause of human gastroenteritis worldwide. Of particular concern is the link between <jats:italic>C. jejuni<\/jats:italic> infections and the subsequent development of Guillain\u2010Barr\u00e9 syndrome, an acquired autoimmune disorder leading to paralysis. All Gram\u2010negative bacteria contain complex glycoconjugates anchored to their outer membranes, but in most strains of <jats:italic>C. jejuni<\/jats:italic>, this lipoglycan lacks the O\u2010antigen repeating units. Recent mass spectrometry analyses indicate that the <jats:italic>C. jejuni<\/jats:italic> 81116 (Penner serotype HS:6) lipoglycan contains two dideoxyhexosamine residues, and enzymological assay data show that this bacterial strain can synthesize both dTDP\u20103\u2010acetamido\u20103,6\u2010dideoxy\u2010<jats:sc>d<\/jats:sc>\u2010glucose and dTDP\u20103\u2010acetamido\u20103,6\u2010dideoxy\u2010<jats:sc>d<\/jats:sc>\u2010galactose. The focus of this investigation is on WlaRG from <jats:italic>C. jejuni<\/jats:italic>, which plays a key role in the production of these unusual sugars by functioning as a pyridoxal 5\u2032\u2010phosphate dependent aminotransferase. Here, we describe the first three\u2010dimensional structures of the enzyme in various complexes determined to resolutions of 1.7 \u00c5 or higher. Of particular significance are the external aldimine structures of WlaRG solved in the presence of either dTDP\u20103\u2010amino\u20103,6\u2010dideoxy\u2010<jats:sc>d<\/jats:sc>\u2010galactose or dTDP\u20103\u2010amino\u20103,6\u2010dideoxy\u2010<jats:sc>d<\/jats:sc>\u2010glucose. These models highlight the manner in which WlaRG can accommodate sugars with differing stereochemistries about their C\u20104\u2032 carbon positions. In addition, we present a corrected structure of WbpE, a related sugar aminotransferase from <jats:italic>Pseudomonas aeruginosa,<\/jats:italic> solved to 1.3 \u00c5 resolution.<\/jats:p>","DOI":"10.1002\/pro.3109","type":"journal-article","created":{"date-parts":[[2016,12,28]],"date-time":"2016-12-28T08:24:58Z","timestamp":1482913498000},"page":"586-599","source":"Crossref","is-referenced-by-count":12,"title":["Structural investigation on WlaRG from <i>Campylobacter jejuni<\/i>: A sugar aminotransferase"],"prefix":"10.1002","volume":"26","author":[{"given":"Garrett T.","family":"Dow","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Wisconsin Madison Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Gilbert","sequence":"additional","affiliation":[{"name":"National Research Council Canada, Human Health Therapeutics Ottawa Ontario K1A 0R6 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James B.","family":"Thoden","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Wisconsin Madison Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hazel M.","family":"Holden","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Wisconsin Madison Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.71.110601.135414"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01429-06"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/BR.30.1.192-255.1966"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.03987.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00912-15"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2cs35438a"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1996.tb00127.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.11.019"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2007.01.006"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2016.09.022"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2012.03.004"},{"key":"e_1_2_7_13_1","first-page":"81116","article-title":"Characterization of the dTDP\u2010Fuc3N and dTDP\u2010Qui3N biosynthetic pathways in","author":"Li ZZ","journal-title":"Campylobacter jejuni"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2010.08.002"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2011.02.003"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi100805b"},{"key":"e_1_2_7_17_1","unstructured":"DeLanoWL(2002) The PyMOL Molecular Graphics System. DeLano Scientific San Carlos CA USA. The PyMOL Molecular Graphics System DeLano Scientific San Carlos CA USA."},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(00)00085-X"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.2745"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00579-08"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M808583200"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(02)00879-1"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3047"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M512987200"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi700751d"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi702430d"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.5b00244"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.062711007"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi8022015"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.21.12111"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(96)90150-0"},{"key":"e_1_2_7_32_1","first-page":"1784","article-title":"Structure of lipopolysaccharides from Gram\u2010negative bacteria. III. Structure of O\u2010specific polysaccharides","volume":"59","author":"Knirel YA","year":"1994","journal-title":"Biokhimiia"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20071044"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505730200"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi501539a"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_2_7_37_1","first-page":"34","article-title":"'DM:' An automated procedure for phase improvement by density modification","volume":"31","author":"Cowtan K","year":"1994","journal-title":"Joint CCP4 ESF\u2010EACBM Newslett Prot Cryst"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444996012255"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889892009944"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi101871f"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.3109","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.3109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T12:01:11Z","timestamp":1694692871000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.3109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,9]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1002\/pro.3109"],"URL":"https:\/\/doi.org\/10.1002\/pro.3109","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,9]]}}}