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The obtained protein was recognized by anti\u2010flagellin and anti\u2010BLS antibodies, keeping the oligomerization capacity of BLS, without affecting the folding of the monomeric protein components determined by circular dichroism. Furthermore, the thermal stability of each fusion partner is conserved, indicating that the interactions that participate in its folding are not affected by the genetic fusion. Besides, either <jats:italic>in vitro<\/jats:italic> or <jats:italic>in vivo<\/jats:italic> using TLR5\u2010deficient animals we could determine that BLS\u2010FliC131 retains the capacity of triggering TLR5. The humoral response against BLS elicited by BLS\u2010FliC131 was stronger than the one elicited by equimolar amounts of BLS\u2009+\u2009FliC. Since BLS scaffold allows the generation of hetero\u2010decameric structures, we expect that flagellin oligomerization on this protein scaffold will generate a new vaccine platform with enhanced capacity to activate immune responses.<\/jats:p>","DOI":"10.1002\/pro.3151","type":"journal-article","created":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T23:32:56Z","timestamp":1488583976000},"page":"1049-1059","source":"Crossref","is-referenced-by-count":13,"title":["Characterization of structural and immunological properties of a fusion protein between flagellin from <i>Salmonella<\/i> and lumazine synthase from <i>Brucella<\/i>"],"prefix":"10.1002","volume":"26","author":[{"given":"Y.","family":"Hiriart","sequence":"first","affiliation":[{"name":"Instituto de Estudios Inmunol\u00f3gicos y Fisiopatol\u00f3gicos (IIFP\u2010CONICET\u2010UNLP) La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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Centre National de la Recherche Scientifique, UMR 8204, Lille, France Universit\u00e9 de Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Rinaldi","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir, IIBBA, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Blanc\u00e1","sequence":"additional","affiliation":[{"name":"Instituto de Estudios Inmunol\u00f3gicos y Fisiopatol\u00f3gicos (IIFP\u2010CONICET\u2010UNLP) La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. C.","family":"Sirard","sequence":"additional","affiliation":[{"name":"Centre d'Infection et d'Immunit\u00e9 de Lille, Institut Pasteur de Lille, Lille, France. Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, U1019, Lille, France. Centre National de la Recherche Scientifique, UMR 8204, Lille, France Universit\u00e9 de Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Goldbaum","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir, IIBBA, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Berguer","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir, IIBBA, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Rumbo","sequence":"additional","affiliation":[{"name":"Instituto de Estudios Inmunol\u00f3gicos y Fisiopatol\u00f3gicos (IIFP\u2010CONICET\u2010UNLP) La Plata 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