{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T06:40:50Z","timestamp":1694846450623},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI056305"],"award-info":[{"award-number":["AI056305"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previously, we determined the crystal structure of apo\u2010TpMglB\u20102, a <jats:sc>d<\/jats:sc>\u2010glucose\u2010binding component of a putative ABC transporter from the syphilis spirochete <jats:italic>Treponema pallidum<\/jats:italic>. The protein had an unusual topology for this class of proteins, raising the question of whether the <jats:sc>d<\/jats:sc>\u2010glucose\u2010binding mode would be different in TpMglB\u20102. Here, we present the crystal structures of a variant of TpMglB\u20102 with and without <jats:sc>d<\/jats:sc>\u2010glucose bound. The structures demonstrate that, despite its aberrant topology, the protein undergoes conformational changes and binds <jats:sc>d<\/jats:sc>\u2010glucose similarly to other Mgl\u2010type proteins, likely facilitating <jats:sc>d<\/jats:sc>\u2010glucose uptake in <jats:italic>T. pallidum<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/pro.3373","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T15:58:01Z","timestamp":1515599881000},"page":"880-885","source":"Crossref","is-referenced-by-count":4,"title":["Crystal stuctures of MglB\u20102 (TP0684), a topologically variant <scp>d<\/scp>\u2010glucose\u2010binding protein from <i>Treponema pallidum,<\/i> reveal a ligand\u2010induced conformational change"],"prefix":"10.1002","volume":"27","author":[{"given":"Chad A.","family":"Brautigam","sequence":"first","affiliation":[{"name":"Department of Biophysics The University of Texas Southwestern Medical Center Dallas Texas 75390"},{"name":"Department of Microbiology The University of Texas Southwestern Medical Center Dallas Texas 75390"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ranjit K.","family":"Deka","sequence":"additional","affiliation":[{"name":"Department of Microbiology The University of Texas Southwestern Medical Center Dallas Texas 75390"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei Z.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Microbiology The University of Texas Southwestern Medical Center Dallas Texas 75390"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael V.","family":"Norgard","sequence":"additional","affiliation":[{"name":"Department of Microbiology The University of Texas Southwestern Medical Center Dallas Texas 75390"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2007.09.005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02484.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1016\/S0021-9258(19)68161-5","article-title":"Hinge\u2010bending in L\u2010Arabinose\u2010 binding protein: the \u201cVenus\u2010flytrap\u201d model","volume":"257","author":"Mao B","year":"1982","journal-title":"J Biol Chem"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"20817","DOI":"10.1016\/S0021-9258(19)47135-4","article-title":"The calcium\u2010binding site in the galactose chemoreceptor protein. Crystallographic and metal\u2010binding studies","volume":"264","author":"Vyas MN","year":"1989","journal-title":"J Biol Chem"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3057628"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.062707807"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0161022"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904026460"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi047302o"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0134(19980201)30:2<144::AID-PROT4>3.0.CO;2-N"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.8.2303-2308.2004"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00182a003"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00622a007"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.141"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5375.375"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-890-0:571"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444906019949"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_2_7_20_1","first-page":"213","article-title":"PHENIX: a comprehensive Python\u2010based system for macromolecular structure determination","volume":"66","author":"Adams PD","year":"2010","journal-title":"Acta Cryst"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.3373","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.3373","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T00:28:11Z","timestamp":1694824091000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.3373"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1002\/pro.3373"],"URL":"https:\/\/doi.org\/10.1002\/pro.3373","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2]]}}}