{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:53:10Z","timestamp":1792673590549,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,7,18]],"date-time":"2018-07-18T00:00:00Z","timestamp":1531872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["321630"],"award-info":[{"award-number":["321630"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human infections by the intracellular bacterial pathogen <jats:italic>Legionella pneumophila<\/jats:italic> result in a severe form of pneumonia, the Legionnaire's disease. <jats:italic>L. pneumophila<\/jats:italic> utilizes a Type IVb secretion (T4bS) system termed \u201c<jats:italic>dot\/icm<\/jats:italic>\u201d to secrete protein effectors to the host cytoplasm. The <jats:italic>dot\/icm<\/jats:italic> system is powered at least in part by a functionally critical AAA+ ATPase, a protein called DotB, thought to belong to the VirB11 family of proteins. Here we present the crystal structure of DotB at 3.19 \u00c5 resolution, in its hexameric form. We observe that DotB is in fact a structural intermediate between VirB11 and PilT family proteins, with a PAS\u2010like N\u2010terminal domain coupled to a RecA\u2010like C\u2010terminal domain. It also shares critical structural elements only found in PilT. The structure also reveals two conformers, termed \u03b1 and \u03b2, with an \u03b1\u03b2\u03b1\u03b2\u03b1\u03b2 configuration. The existence of \u03b1 and \u03b2 conformers in this class of proteins was confirmed by solving the structure of DotB from another bacterial pathogen, Yersinia, where, intriguingly, we observed an \u03b1\u03b1\u03b2\u03b1\u03b1\u03b2 configuration. The two conformers co\u2010exist regardless of the nucleotide\u2010bound states of the proteins. Our investigation therefore reveals that these ATPases can adopt a wider range of conformational states than was known before, shedding new light on the extraordinary spectrum of conformations these ATPases can access to carry out their function. Overall, the structure of DotB provides a template for further rational drug design to develop more specific antibiotics to tackle Legionnaire's disease.<\/jats:p><jats:p>PDB Code(s): <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/firstglance.jmol.org\/fg.htm?mol=Will\">Will<\/jats:ext-link>; <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/firstglance.jmol.org\/fg.htm?mol=be\">be<\/jats:ext-link>; <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/firstglance.jmol.org\/fg.htm?mol=provided\">provided<\/jats:ext-link><\/jats:p>","DOI":"10.1002\/pro.3439","type":"journal-article","created":{"date-parts":[[2018,5,17]],"date-time":"2018-05-17T08:41:02Z","timestamp":1526546462000},"page":"1464-1475","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["X\u2010ray crystal structures of the type IVb secretion system DotB ATPases"],"prefix":"10.1002","volume":"27","author":[{"given":"Marie S.","family":"Prevost","sequence":"first","affiliation":[{"name":"Institute of Structural and Molecular Biology, University College London and Birkbeck, Malet Street London WC1E 7HX United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Waksman","sequence":"additional","affiliation":[{"name":"Institute of Structural and Molecular Biology, University College London and Birkbeck, Malet Street London WC1E 7HX United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1056\/NEJM197712012972202","article-title":"Legionnaires' disease","volume":"297","author":"McDade JE","year":"1977","journal-title":"N Engl J 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