{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T01:26:49Z","timestamp":1771032409162,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,7,18]],"date-time":"2018-07-18T00:00:00Z","timestamp":1531872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM115921 to H. M. H."],"award-info":[{"award-number":["GM115921 to H. M. H."]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005440","name":"Center for Scientific Review","doi-asserted-by":"publisher","award":["GM115921"],"award-info":[{"award-number":["GM115921"]}],"id":[{"id":"10.13039\/100005440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent studies have demonstrated that the O\u2010antigens of some pathogenic bacteria such as <jats:italic>Brucella abortus<\/jats:italic>, <jats:italic>Francisella tularensis<\/jats:italic>, and <jats:italic>Campylobacter jejuni<\/jats:italic> contain quite unusual <jats:italic>N<\/jats:italic>\u2010formylated sugars (3\u2010formamido\u20103,6\u2010dideoxy\u2010<jats:sc>d<\/jats:sc>\u2010glucose or 4\u2010formamido\u20104,6\u2010dideoxy\u2010<jats:sc>d<\/jats:sc>\u2010glucose). Typically, four enzymes are required for the formation of such sugars: a thymidylyltransferase, a 4,6\u2010dehydratase, a pyridoxal 5'\u2010phosphate or PLP\u2010dependent aminotransferase, and an <jats:italic>N<\/jats:italic>\u2010formyltransferase. To date, there have been no published reports of <jats:italic>N<\/jats:italic>\u2010formylated sugars associated with <jats:italic>Mycobacterium tuberculosis<\/jats:italic>. A recent investigation from our laboratories, however, has demonstrated that one gene product from <jats:italic>M. tuberculosis<\/jats:italic>, Rv3404c, functions as a sugar <jats:italic>N<\/jats:italic>\u2010formyltransferase. Given that <jats:italic>M. tuberculosis<\/jats:italic> produces <jats:sc>l<\/jats:sc>\u2010rhamnose, both a thymidylyltransferase (Rv0334) and a 4,6\u2010dehydratase (Rv3464) required for its formation have been identified. Thus, there is one remaining enzyme needed for the production of an <jats:italic>N<\/jats:italic>\u2010formylated sugar in <jats:italic>M. tuberculosis<\/jats:italic>, namely a PLP\u2010dependent aminotransferase. Here we demonstrate that the <jats:italic>M. tuberculosis rv3402c<\/jats:italic> gene encodes such an enzyme. Our data prove that <jats:italic>M. tuberculosis<\/jats:italic> contains all of the enzymatic activities required for the formation of dTDP\u20104\u2010formamido\u20104,6\u2010dideoxy\u2010<jats:sc>d<\/jats:sc>\u2010glucose. Indeed, the <jats:italic>rv3402c<\/jats:italic> gene product likely contributes to virulence or persistence during infection, though its temporal expression and location remain to be determined.<\/jats:p>","DOI":"10.1002\/pro.3443","type":"journal-article","created":{"date-parts":[[2018,5,15]],"date-time":"2018-05-15T08:52:12Z","timestamp":1526374332000},"page":"1491-1497","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The <i>Mycobacterium tuberculosis<\/i> complex has a pathway for the biosynthesis of 4\u2010formamido\u20104,6\u2010dideoxy\u2010<scp>d<\/scp>\u2010glucose"],"prefix":"10.1002","volume":"27","author":[{"given":"Haley A.","family":"Brown","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Wisconsin Madison Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evgeny","family":"Vinogradov","sequence":"additional","affiliation":[{"name":"Human Health Therapeutics, National Research Council Canada, 100 Sussex Drive Ottawa Ontario K1A OR6 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Gilbert","sequence":"additional","affiliation":[{"name":"Human Health Therapeutics, National Research Council Canada, 100 Sussex Drive Ottawa Ontario K1A OR6 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hazel M.","family":"Holden","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Wisconsin Madison Wisconsin 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