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A vaccine against <jats:italic>Chlamydia<\/jats:italic> is highly desirable but currently not available. MOMP accounts for ~ 60% of the chlamydial protein mass and is considered to be one of the lead vaccine candidates against <jats:italic>C. trachomatis<\/jats:italic>. We report on the spectroscopic analysis of <jats:italic>C. trachomatis<\/jats:italic> native MOMP Serovars D, E, F, and J as well as <jats:italic>C. muridarum<\/jats:italic> MOMP by size exclusion chromatography multi angle light scattering (SEC MALS), circular dichroism (CD) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR\u2010FTIR). MOMP was purified from the native bacterium grown in either adherent HeLa cells or in different suspension cell lines. Our results confirm that MOMP forms homo\u2010trimers in detergent micelles. The secondary structure composition of <jats:italic>C. trachomatis<\/jats:italic> MOMP was conserved across serovars, but different from composition of <jats:italic>C. muridarum<\/jats:italic> MOMP with a 13% (CD) to 18% (ATR\u2010FTIR) reduction in \u03b2\u2010sheet conformation for <jats:italic>C. trachomatis<\/jats:italic> MOMP. When Serovar E MOMP was isolated from suspension cell lines the \u03b1\u2010helix content increased by 7% (CD) to 13% (ATIR\u2010FTIR). Maintenance of a native\u2010like tertiary and quaternary structure in subunit vaccines is important for the generation of protective antibodies. This biophysical characterization of MOMP presented here serves, in the absence of functional assays, as a method for monitoring the structural integrity of MOMP.<\/jats:p>","DOI":"10.1002\/pro.3501","type":"journal-article","created":{"date-parts":[[2018,8,25]],"date-time":"2018-08-25T07:19:40Z","timestamp":1535181580000},"page":"1923-1941","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Spectroscopic analysis of chlamydial major outer membrane protein in support of structure elucidation"],"prefix":"10.1002","volume":"27","author":[{"given":"Robert W.","family":"Hepler","sequence":"first","affiliation":[{"name":"Infectious Diseases and Vaccines Discovery MRL, Merck &amp; Co., Inc.  Kenilworth New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debbie D.","family":"Nahas","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Vaccines Discovery MRL, Merck &amp; Co., Inc.  Kenilworth New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bob","family":"Lucas","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Vaccines Discovery MRL, Merck &amp; Co., Inc.  Kenilworth New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"Kaufhold","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Vaccines Discovery MRL, Merck &amp; Co., Inc.  Kenilworth New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica A","family":"Flynn","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Vaccines Discovery MRL, Merck &amp; Co., Inc.  Kenilworth New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer D.","family":"Galli","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Vaccines Discovery MRL, Merck &amp; Co., Inc.  Kenilworth New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan","family":"Swoyer","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Vaccines Discovery MRL, Merck &amp; Co., Inc.  Kenilworth New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Vaccine Process Development MRL, Merck &amp; Co., Inc.  Kenilworth New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy S.","family":"Espeseth","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Vaccines Discovery MRL, Merck &amp; Co., Inc.  Kenilworth New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph G.","family":"Joyce","sequence":"additional","affiliation":[{"name":"Vaccine Process Development MRL, Merck &amp; Co., Inc.  Kenilworth New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"Cook","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Vaccines Discovery MRL, Merck &amp; Co., Inc.  Kenilworth New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eberhard","family":"Durr","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Vaccines Discovery MRL, Merck &amp; Co., Inc.  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