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This study is a comprehensive analysis of 40 ABH enzyme families focusing on two identified substructures: the nucleophile zone and the oxyanion zone, which co\u2010ordinate the catalytic nucleophile and the residues of the oxyanion hole, and independently reported as critical for the enzymatic activity. We also frequently observed an aromatic cluster near the nucleophile and oxyanion zones, and opposite the ligand\u2010binding site. The nucleophile zone, the oxyanion zone and the residue cluster enriched in aromatic side chains comprise a three\u2010dimensional structural organization that shapes the active site of ABH enzymes and plays an important role in the enzymatic function by structurally stabilizing the catalytic nucleophile and the residues of the oxyanion hole. The structural data support the notion that the aromatic cluster can participate in co\u2010ordination of the catalytic histidine loop, and properly place the catalytic histidine next to the catalytic nucleophile.<\/jats:p>","DOI":"10.1002\/pro.3527","type":"journal-article","created":{"date-parts":[[2018,10,12]],"date-time":"2018-10-12T13:15:38Z","timestamp":1539350138000},"page":"344-364","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Distinctive structural motifs co\u2010ordinate the catalytic nucleophile and the residues of the oxyanion hole in the alpha\/beta\u2010hydrolase fold enzymes"],"prefix":"10.1002","volume":"28","author":[{"given":"Polytimi S.","family":"Dimitriou","sequence":"first","affiliation":[{"name":"Structural Bioinformatics Laboratory, Biochemistry, Faculty of Science and Engineering \u00c5bo Akademi University  Turku 20520 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