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Bacillithiol is produced via a three\u2010enzyme pathway that includes the action of the zinc\u2010dependent deacetylase BshB. Previous studies identified conserved aspartate and histidine residues within the active site that are involved in metal binding and catalysis, but the enzymatic mechanism is not fully understood. Here we report two X\u2010ray crystallographic structures of BshB from <jats:italic>Bacillus subtilis<\/jats:italic> that provide insight into the BshB catalytic mechanism.<\/jats:p>","DOI":"10.1002\/pro.3808","type":"journal-article","created":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T02:31:17Z","timestamp":1577068277000},"page":"1021-1025","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["X\u2010ray crystallographic structure of BshB, the zinc\u2010dependent deacetylase involved in bacillithiol biosynthesis"],"prefix":"10.1002","volume":"29","author":[{"given":"Robert L.","family":"Woodward","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Mount Union  Alliance Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaela M.","family":"Castleman","sequence":"additional","affiliation":[{"name":"Department of Chemistry Grand Valley State University  Allendale Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chelsea E.","family":"Meloche","sequence":"additional","affiliation":[{"name":"Department of Chemistry Grand Valley State University  Allendale Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary E.","family":"Karpen","sequence":"additional","affiliation":[{"name":"Department of Chemistry Grand Valley State University  Allendale Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clare G.","family":"Carlson","sequence":"additional","affiliation":[{"name":"Department of Chemistry Grand Valley State University  Allendale Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William H.","family":"Yobi","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Mount Union  Alliance Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline 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