{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T10:01:31Z","timestamp":1756461691544,"version":"3.40.5"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T00:00:00Z","timestamp":1580342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["HHSN272201200026C","HHSN272201700060C"],"award-info":[{"award-number":["HHSN272201200026C","HHSN272201700060C"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Acyl\u2010coenzyme A\u2010dependent <jats:italic>N<\/jats:italic>\u2010acetyltransferases (AACs) catalyze the modification of aminoglycosides rendering the bacteria carrying such enzymes resistant to this class of antibiotics. Here we present the crystal structure of AAC(3)\u2010Ia enzyme from <jats:italic>Serratia marcescens<\/jats:italic> in complex with coenzyme A determined to 1.8\u2009\u00c5 resolution. This enzyme served as an architype for the AAC enzymes targeting the amino group at Position 3 of aminoglycoside main aminocyclitol ring. The structure of this enzyme has been previously determined only in truncated form and was interpreted as distinct from subsequently characterized AACs. The reason for the unusual arrangement of secondary structure elements of AAC(3)\u2010Ia was not further investigated. By determining the full\u2010length structure of AAC(3)\u2010Ia we establish that this enzyme adopts the canonical AAC fold conserved across this family and it does not undergo through significant rearrangement of secondary structure elements upon ligand binding as was proposed previously. In addition, our results suggest that the C\u2010terminal tail in AAC(3)\u2010Ia monomer forms intramolecular hydrogen bonds that contributes to formation of stable dimer, representing the predominant oligomeric state for this enzyme.<\/jats:p>","DOI":"10.1002\/pro.3811","type":"journal-article","created":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T11:51:32Z","timestamp":1577361092000},"page":"803-808","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Structure of the full\u2010length <i>Serratia marcescens<\/i> acetyltransferase AAC(3)\u2010Ia in complex with coenzyme A"],"prefix":"10.1002","volume":"29","author":[{"given":"Georgy","family":"Popov","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology and Infectious Diseases University of Calgary  Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Evdokimova","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Applied Chemistry University of Toronto  Toronto Ontario Canada"},{"name":"Center for Structural Genomics for Infectious Diseases University of Calgary  Calgary Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Stogios","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Applied Chemistry University of Toronto  Toronto Ontario Canada"},{"name":"Center for Structural Genomics for Infectious Diseases University of Calgary  Calgary Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5256-9237","authenticated-orcid":false,"given":"Alexei","family":"Savchenko","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Infectious Diseases University of Calgary  Calgary Alberta Canada"},{"name":"Department of Chemical Engineering and Applied Chemistry University of Toronto  Toronto Ontario Canada"},{"name":"Center for Structural Genomics for Infectious Diseases University of Calgary  Calgary 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