{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:09:14Z","timestamp":1790629754155,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T00:00:00Z","timestamp":1580169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["HHSN272201200025C"],"award-info":[{"award-number":["HHSN272201200025C"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["HHSN272201700059C"],"award-info":[{"award-number":["HHSN272201700059C"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Neisseria gonorrhoeae<\/jats:italic><\/jats:styled-content> (<jats:italic>Ng<\/jats:italic>) and <jats:italic>Chlamydia trachomatis<\/jats:italic> (<jats:italic>Ct<\/jats:italic>) are the most commonly reported sexually transmitted bacteria worldwide and usually present as co\u2010infections. Increasing resistance of <jats:italic>Ng<\/jats:italic> to currently recommended dual therapy of azithromycin and ceftriaxone presents therapeutic challenges for syndromic management of <jats:italic>Ng<\/jats:italic>\u2010<jats:italic>Ct<\/jats:italic> co\u2010infections. Development of a safe, effective, and inexpensive dual therapy for <jats:italic>Ng<\/jats:italic>\u2010<jats:italic>Ct<\/jats:italic> co\u2010infections is an effective strategy for the global control and prevention of these two most prevalent bacterial sexually transmitted infections. Glyceraldehyde\u20103\u2010phosphate dehydrogenase (GAPDH) is a validated drug target with two approved drugs for indications other than antibacterials. Nonetheless, any new drugs targeting GAPDH in <jats:italic>Ng<\/jats:italic> and <jats:italic>Ct<\/jats:italic> must be specific inhibitors of bacterial GAPDH that do not inhibit human GAPDH, and structural information of <jats:italic>Ng<\/jats:italic> and <jats:italic>Ct<\/jats:italic> GAPDH will aid in finding such selective inhibitors. Here, we report the X\u2010ray crystal structures of <jats:italic>Ng<\/jats:italic> and <jats:italic>Ct<\/jats:italic> GAPDH. Analysis of the structures demonstrates significant differences in amino acid residues in the active sites of human GAPDH from those of the two bacterial enzymes suggesting design of compounds to selectively inhibit <jats:italic>Ng<\/jats:italic> and <jats:italic>Ct<\/jats:italic> is possible. We also describe an efficient in vitro assay of recombinant GAPDH enzyme activity amenable to high\u2010throughput drug screening to aid in identifying inhibitory compounds and begin to address selectivity.<\/jats:p>","DOI":"10.1002\/pro.3824","type":"journal-article","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T04:43:11Z","timestamp":1578890591000},"page":"768-778","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Structures of glyceraldehyde 3\u2010phosphate dehydrogenase in <scp><i>Neisseria gonorrhoeae<\/i><\/scp> and <i>Chlamydia trachomatis<\/i>"],"prefix":"10.1002","volume":"29","author":[{"given":"Kayleigh F.","family":"Barrett","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Allergy and Infectious Diseases Center for Emerging and Re\u2010emerging Infectious Diseases (CERID), University of Washington  Seattle Washington"},{"name":"Seattle Structural Genomics 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