{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T12:27:33Z","timestamp":1750249653992,"version":"3.40.5"},"reference-count":52,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T00:00:00Z","timestamp":1604016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DE\u2010AC02\u201006CH11357","P30 GM124165"],"award-info":[{"award-number":["DE\u2010AC02\u201006CH11357","P30 GM124165"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1129928"],"award-info":[{"award-number":["APP1129928"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glyceraldehyde 3\u2010phosphate dehydrogenase (GAPDH) is an evolutionarily conserved essential enzyme in the glycolytic pathway. GAPDH is also involved in a wide spectrum of non\u2010catalytic cellular \u2018moonlighting\u2019 functions. Bacterial surface\u2010associated GAPDHs engage in many host interactions that aid in colonization, pathogenesis, and virulence. We have structurally and functionally characterized the recombinant GAPDH of the obligate intracellular bacteria <jats:italic>Chlamydia trachomatis<\/jats:italic>, the leading cause of sexually transmitted bacterial and ocular infections. Contrary to earlier speculations, recent data confirm the presence of glucose\u2010catabolizing enzymes including GAPDH in both stages of the biphasic life cycle of the bacterium. The high\u2010resolution crystal structure described here provides a close\u2010up view of the enzyme's active site and surface topology and reveals two chemically modified cysteine residues. Moreover, we show for the first time that purified <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis<\/jats:italic> GAPDH binds to human plasminogen and plasmin. Based on the versatility of GAPDH's functions, data presented here emphasize the need for investigating the <jats:italic>Chlamydiae<\/jats:italic> GAPDH's involvement in biological functions beyond energy metabolism.<\/jats:p>","DOI":"10.1002\/pro.3975","type":"journal-article","created":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T13:30:42Z","timestamp":1602768642000},"page":"2446-2458","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp><i>Chlamydia trachomatis<\/i><\/scp> glyceraldehyde 3\u2010phosphate dehydrogenase: Enzyme kinetics, high\u2010resolution crystal structure, and plasminogen binding"],"prefix":"10.1002","volume":"29","author":[{"given":"Norbert","family":"Schormann","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Alabama at Birmingham  Birmingham Alabama USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Campos","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics Birmingham\u2010Southern College  Birmingham Alabama USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachael","family":"Motamed","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics Birmingham\u2010Southern College  Birmingham Alabama USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine L.","family":"Hayden","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics Birmingham\u2010Southern College  Birmingham Alabama USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph R.","family":"Gould","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Alabama at Birmingham  Birmingham Alabama USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todd J.","family":"Green","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Alabama at Birmingham  Birmingham Alabama USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Senkovich","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics Midwestern University  Glendale Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surajit","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Northeastern Collaborative Access Team and Department of Chemistry and Chemical Biology Cornell University  Argonne Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glen C.","family":"Ulett","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, and Menzies Health Institute Queensland Griffith University  Parklands Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5262-9140","authenticated-orcid":false,"given":"Debasish","family":"Chattopadhyay","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Alabama at Birmingham  Birmingham Alabama USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0149011"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"F1000 Faculty R","DOI":"10.12688\/f1000research.18832.1","article-title":"A renewed tool kit to explore chlamydia pathogenesis: From molecular genetics to new infection models","volume":"8","author":"Dolat L","year":"2019","journal-title":"F1000Res"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kws583"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-1030-9"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00070"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01464.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm9802620"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201600982R"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.2.415"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03390.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1139\/w03-042"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.3.1379"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2010.08.004"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0063244"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M513408200"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.423731"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00157-09"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.2416-2419.2004"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2014.08.017"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0144196"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.4.2459"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1991.tb16032.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.13237"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6807-9-9"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2019.01.010"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.00641.x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00046a004"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3824"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(73)90388-4"},{"key":"e_1_2_8_31_1","first-page":"290","article-title":"High\u2010resolution structure of human D\u2010glyceraldehyde\u20103\u2010phosphate dehydrogenase","volume":"62","author":"Jenkins JL","year":"2006","journal-title":"Acta Cryst"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00182749"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M805287200"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2019102140"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2006.01.012"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0508412103"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2014-0295"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0165917"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.764209"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-800255-1.00005-3"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.169"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl206"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2012.02.012"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.05.022"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01218-06"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002363"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909047337"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910045749"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444996012255"},{"key":"e_1_2_8_51_1","first-page":"352","article-title":"Towards automated crystallographic structure refinement with phenix.Refine","volume":"68","author":"Afonine PV","year":"2012","journal-title":"Acta Cryst"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909042073"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.3975","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pro.3975","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.3975","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T06:15:52Z","timestamp":1693808152000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.3975"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,30]]},"references-count":52,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["10.1002\/pro.3975"],"URL":"https:\/\/doi.org\/10.1002\/pro.3975","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"type":"print","value":"0961-8368"},{"type":"electronic","value":"1469-896X"}],"subject":[],"published":{"date-parts":[[2020,10,30]]},"assertion":[{"value":"2020-08-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}