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In the bioluminescent marine bacterium\n                    <jats:italic>Shewanella woodyi<\/jats:italic>\n                    (\n                    <jats:italic>Sw<\/jats:italic>\n                    ), H\u2010NOX proteins regulate quorum sensing and biofilm formation. In higher animals, soluble guanylyl cyclase (sGC) binds nitric oxide with an H\u2010NOX domain to induce cyclase activity and regulate vascular tone, wound healing and memory formation. sGC also binds stimulator compounds targeting cardiovascular disease. The molecular details of stimulator binding to sGC remain obscure but involve a binding pocket near an interface between H\u2010NOX and coiled\u2010coil domains. Here, we report the full NMR structure for CO\u2010ligated\n                    <jats:italic>Sw<\/jats:italic>\n                    H\u2010NOX in the presence and absence of stimulator compound IWP\u2010051, and its backbone dynamics. Nonplanar heme geometry was retained using a semi\u2010empirical quantum potential energy approach. Although IWP\u2010051 binding is weak, a single binding conformation was found at the interface of the two H\u2010NOX subdomains, near but not overlapping with sites identified in sGC. Binding leads to rotation of the subdomains and closure of the binding pocket. Backbone dynamics are similar across both domains except for two helix\u2010connecting loops, which display increased dynamics that are further enhanced by compound binding. Structure\u2010based sequence analyses indicate high sequence diversity in the binding pocket, but the pocket itself appears conserved among H\u2010NOX proteins. The largest dynamical loop lies at the interface between\n                    <jats:italic>Sw<\/jats:italic>\n                    H\u2010NOX and its binding partner as well as in the interface with the coiled coil in sGC, suggesting a critical role for the loop in signal transduction.\n                  <\/jats:p>","DOI":"10.1002\/pro.4005","type":"journal-article","created":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T09:23:47Z","timestamp":1606296227000},"page":"448-463","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Solution structures of the\n                    <i>Shewanella woodyi<\/i>\n                    <scp>H\u2010NOX<\/scp>\n                    protein in the presence and absence of soluble guanylyl cyclase stimulator\n                    <scp>IWP<\/scp>\n                    \u2010051"],"prefix":"10.1002","volume":"30","author":[{"given":"Cheng\u2010Yu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Arizona  Tucson Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woonghee","family":"Lee","sequence":"additional","affiliation":[{"name":"National Magnetic Resonance Facility at Madison, Biochemistry Department University of Wisconsin\u2010Madison  Madison Wisconsin USA"},{"name":"Department of Chemistry University of Colorado Denver  Denver Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul A.","family":"Renhowe","sequence":"additional","affiliation":[{"name":"Cyclerion Therapeutics  Cambridge Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joon","family":"Jung","sequence":"additional","affiliation":[{"name":"Cyclerion Therapeutics  Cambridge Massachusetts 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