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This bacterium can be found in freshwater environments as a free\u2010living organism, but it is also an intracellular parasite of protozoa. Human infection occurs when inhaled aerosolized pathogen comes into contact with the alveolar mucosa and replicates in alveolar macrophages. <jats:italic>Legionella<\/jats:italic> enters the host cell by phagocytosis and redirects the <jats:italic>Legionella<\/jats:italic>\u2010containing phagosomes from the phagocytic maturation pathway. These nascent phagosomes fuse with ER\u2010derived secretory vesicles and membranes forming the <jats:italic>Legionella<\/jats:italic>\u2010containing vacuole. <jats:italic>Legionella<\/jats:italic> subverts many host cellular processes by secreting over 300 effector proteins into the host cell via the Dot\/Icm type IV secretion system. The cellular function for many Dot\/Icm effectors is still unknown. Here, we present a structural and functional study of <jats:italic>L<\/jats:italic>. <jats:italic>pneumophila<\/jats:italic> effector RavA (Lpg0008). Structural analysis revealed that the RavA consists of four ~85 residue long \u03b1\u2010helical domains with similar folds, which show only a low level of structural similarity to other protein domains. The ~90 residues long C\u2010terminal segment is predicted to be natively unfolded. We show that during <jats:italic>L. pneumophila<\/jats:italic> infection of human cells, RavA localizes to the Golgi apparatus and to the plasma membrane. The same localization is observed when RavA is expressed in human cells. The localization signal resides within the C\u2010terminal sequence C<jats:sup>409<\/jats:sup>WTSFCGLF<jats:sup>417<\/jats:sup>. Yeast\u2010two\u2010hybrid screen using RavA as bait identified RAB11A as a potential binding partner. RavA is present in <jats:italic>L. pneumophila<\/jats:italic> strains but only distant homologs are found in other <jats:italic>Legionella<\/jats:italic> species, where the number of repeats varies.<\/jats:p>","DOI":"10.1002\/pro.4057","type":"journal-article","created":{"date-parts":[[2021,3,4]],"date-time":"2021-03-04T06:00:43Z","timestamp":1614837643000},"page":"940-955","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Structural and functional study of <i>Legionella pneumophila<\/i> effector RavA"],"prefix":"10.1002","volume":"30","author":[{"given":"Ivy Y. 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