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However, many aspects of the signaling mechanism remain elusive, including oligomeric association and clustering of B7\u20102 on the cell surface. Here, we describe the structure of the IgV domain of B7\u20102 and its cryptic association into 1D arrays that appear to represent the pre\u2010signaling state of B7\u20102 on the cell membrane. Super\u2010resolution microscopy experiments on heterologous cells expressing B7\u20102 and B7\u20101 suggest, B7\u20102 form relatively elongated and larger clusters compared to B7\u20101. The sequence and structural comparison of other B7 family members, B7\u20101:CTLA4 and B7\u20102:CTLA\u20104 complex structures, support our view that the observed B7\u20102 1D zipper array is physiologically important. This observed 1D zipper\u2010like array also provides an explanation for its clustering, and upright orientation on the cell surface, and avoidance of spurious signaling.<\/jats:p>","DOI":"10.1002\/pro.4151","type":"journal-article","created":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T08:00:25Z","timestamp":1625126425000},"page":"1958-1973","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cryptic association of <scp>B7<\/scp>\u20102 molecules and its implication for clustering"],"prefix":"10.1002","volume":"30","author":[{"given":"Swetha","family":"Lankipalli","sequence":"first","affiliation":[{"name":"Biological Sciences Division Poornaprajna Institute of Scientific Research (PPISR)  Bengaluru India"},{"name":"Manipal Academy of Higher Education  Manipal Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahadeva Swamy","family":"H S","sequence":"additional","affiliation":[{"name":"Centre for Neuroscience Indian Institute of Science  Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deepak","family":"Selvam","sequence":"additional","affiliation":[{"name":"Jawaharlal Nehru Center for Advance Scientific Research  Bengaluru Karnataka India"},{"name":"National Institute for Research in Tuberculosis  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5733-6694","authenticated-orcid":false,"given":"Dibyendu","family":"Samanta","sequence":"additional","affiliation":[{"name":"School of Bioscience, Sir J. 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