{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T01:12:21Z","timestamp":1775869941878,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T00:00:00Z","timestamp":1638230400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI056305"],"award-info":[{"award-number":["AI056305"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["S10OD025018"],"award-info":[{"award-number":["S10OD025018"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Antibiotic resistance is a challenge for the control of bacterial infections. In an effort to explore unconventional avenues for antibacterial drug development, we focused on the FMN\u2010transferase activity of the enzyme Ftp from the syphilis spirochete, <jats:italic>Treponema pallidum<\/jats:italic> (Ftp_Tp). This enzyme, which is only found in prokaryotes and trypanosomatids, post\u2010translationally modifies proteins in the periplasm, covalently linking FMN (from FAD) to proteins that typically are important for establishing an essential electrochemical gradient across the cytoplasmic membrane. As such, Ftp inhibitors potentially represent a new class of antimicrobials. Previously, we showed that AMP is both a product of the Ftp_tp\u2010catalyzed reaction and an inhibitor of the enzyme. As a preliminary step in exploiting this property to develop a novel Ftp_Tp inhibitor, we have used structural and solution studies to examine the inhibitory and enzyme\u2010binding properties of several adenine\u2010based nucleosides, with particular focus on the 2\u2010position of the purine ring. Implications for future drug design are discussed.<\/jats:p>","DOI":"10.1002\/pro.4241","type":"journal-article","created":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T08:45:55Z","timestamp":1637311555000},"page":"545-551","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Inhibition of bacterial <scp>FMN<\/scp> transferase: A potential avenue for countering antimicrobial resistance"],"prefix":"10.1002","volume":"31","author":[{"given":"Ranjit K.","family":"Deka","sequence":"first","affiliation":[{"name":"Department of Microbiology UT Southwestern Medical Center  Dallas Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akanksha","family":"Deka","sequence":"additional","affiliation":[{"name":"Plano East Senior High School  Plano Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei Z.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Microbiology UT Southwestern Medical Center  Dallas Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael V.","family":"Norgard","sequence":"additional","affiliation":[{"name":"Department of Microbiology UT Southwestern Medical Center  Dallas Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6563-1338","authenticated-orcid":false,"given":"Chad A.","family":"Brautigam","sequence":"additional","affiliation":[{"name":"Department of Microbiology UT Southwestern Medical Center  Dallas Texas USA"},{"name":"Department of Biophysics UT Southwestern Medical Center  Dallas Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00519-15"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.141"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00615-12"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.449975"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0498-z"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.455402"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0555-8"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00401"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10863-014-9565-9"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2009.12.020"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02249-20"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201759200"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14003"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.306"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20180524"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1914939117"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA120.014229"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1915678116"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11216-7"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5375.375"},{"key":"e_1_2_8_22_1","unstructured":"U.S. Centers for Disease Control and Prevention.National action plan for combating antibiotic\u2010resistant bacteria 2020\u20132025; 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