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These incidents often result in death due to paralysis caused by \u03b1\u2010neurotoxins from the three\u2010finger toxin (3FTx) family, which are abundant in elapid venoms. Due to their small size, 3FTxs are among the snake toxins that are most poorly neutralized by current antivenoms, which are based on polyclonal antibodies of equine or ovine origin. While antivenoms have saved countless lives since their development in the late 18th century, an opportunity now exists to improve snakebite envenoming therapy via the application of new biotechnological methods, particularly by developing monoclonal antibodies against poorly neutralized \u03b1\u2010neurotoxins. Here, we describe the use of phage\u2010displayed synthetic antibody libraries and the development and characterization of six synthetic antibodies built on a human IgG framework and developed against \u03b1\u2010cobratoxin \u2013 the most abundant long\u2010chain \u03b1\u2010neurotoxin from <jats:italic>Naja kaouthia<\/jats:italic> venom. The synthetic antibodies exhibited sub\u2010nanomolar affinities to \u03b1\u2010cobratoxin and neutralized the curare\u2010mimetic effect of the toxin <jats:italic>in vitro<\/jats:italic>. These results demonstrate that phage display technology based on synthetic repertoires can be used to rapidly develop human antibodies with drug\u2010grade potencies as inhibitors of venom toxins.<\/jats:p>","DOI":"10.1002\/pro.4296","type":"journal-article","created":{"date-parts":[[2022,4,9]],"date-time":"2022-04-09T15:00:07Z","timestamp":1649516407000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Synthetic antibodies block receptor binding and current\u2010inhibiting effects of \u03b1\u2010cobratoxin from <i>Naja kaouthia<\/i>"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4785-4508","authenticated-orcid":false,"given":"Shane","family":"Miersch","sequence":"first","affiliation":[{"name":"The Donnelly Centre University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"de la 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