{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T14:48:36Z","timestamp":1790347716832,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T00:00:00Z","timestamp":1651017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Aromatic residues appeared relatively late in the evolution of protein sequences to stabilize the globular proteins' folding core and are less in the intrinsically disordered regions (IDRs). Recent advances in protein liquid\u2013liquid phase separation (LLPS) studies have also shown that aromatic residues in IDRs often act as \u201cstickers\u201d to promote multivalent interactions in forming higher\u2010order oligomers. To study how general these structure\u2010promoting residues are in IDRs, we compared levels of sequence disorder in RNA binding proteins (RBPs), which are often found to undergo LLPS, and the human proteome. We found that aromatic residues appear more frequently than expected in the IDRs of RBPs and, through multiple sequence alignment analysis, those aromatic residues are often conserved among chordates. Using TDP\u201043, FUS, and some other well\u2010studied LLPS proteins as examples, the conserved aromatic residues are important to their LLPS\u2010related functions. These analyses suggest that aromatic residues may have contributed twice to evolution: stabilizing structured proteins and assembling biomolecular condensates.<\/jats:p>","DOI":"10.1002\/pro.4317","type":"journal-article","created":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T06:49:10Z","timestamp":1651042150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["The return of the rings: Evolutionary convergence of aromatic residues in the intrinsically disordered regions of RNA\u2010binding proteins for liquid\u2013liquid phase separation"],"prefix":"10.1002","volume":"31","author":[{"given":"Wen\u2010Lin","family":"Ho","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, National Yang Ming Chiao Tung University  Taipei 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