{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:37:11Z","timestamp":1788871031389,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2010,7,28]],"date-time":"2010-07-28T00:00:00Z","timestamp":1280275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Office of Science (BER), US Department of Energy","award":["DE-FG02-07ER64501"],"award-info":[{"award-number":["DE-FG02-07ER64501"]}]},{"name":"Office of Science (BER), US Department of Energy","award":["DE-FC03-02ER63421"],"award-info":[{"award-number":["DE-FC03-02ER63421"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["5R01 GM075993"],"award-info":[{"award-number":["5R01 GM075993"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100016198","name":"Industrial Macromolecular Crystallography Association","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100016198","id-type":"DOI","asserted-by":"crossref"}]},{"name":"US Department of Energy Office of Science Office of Basic Energy Sciences","award":["DE-AC02-06CH11357"],"award-info":[{"award-number":["DE-AC02-06CH11357"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The 50\u2010residue snake venom protein\n                    <jats:sc>L<\/jats:sc>\n                    \u2010omwaprin and its enantiomer\n                    <jats:sc>D<\/jats:sc>\n                    \u2010omwaprin were prepared by total chemical synthesis. Radial diffusion assays were performed against\n                    <jats:italic>Bacillus megaterium<\/jats:italic>\n                    and\n                    <jats:italic>Bacillus anthracis<\/jats:italic>\n                    ; both\n                    <jats:sc>L<\/jats:sc>\n                    \u2010 and\n                    <jats:sc>D<\/jats:sc>\n                    \u2010omwaprin showed antibacterial activity against\n                    <jats:italic>B. megaterium<\/jats:italic>\n                    . The native protein enantiomer, made of\n                    <jats:sc>L<\/jats:sc>\n                    \u2010amino acids, failed to crystallize readily. However, when a racemic mixture containing equal amounts of\n                    <jats:sc>L<\/jats:sc>\n                    \u2010 and\n                    <jats:sc>D<\/jats:sc>\n                    \u2010omwaprin was used, diffraction quality crystals were obtained. The racemic protein sample crystallized in the centrosymmetric space group P2\n                    <jats:sub>1<\/jats:sub>\n                    \/c and its structure was determined at atomic resolution (1.33 \u00c5) by a combination of Patterson and direct methods based on the strong scattering from the sulfur atoms in the eight cysteine residues per protein. Racemic crystallography once again proved to be a valuable method for obtaining crystals of recalcitrant proteins and for determining high\u2010resolution X\u2010ray structures by direct methods.\n                  <\/jats:p>","DOI":"10.1002\/pro.468","type":"journal-article","created":{"date-parts":[[2010,7,28]],"date-time":"2010-07-28T09:41:59Z","timestamp":1280310119000},"page":"1840-1849","source":"Crossref","is-referenced-by-count":53,"title":["Determination of the X\u2010ray structure of the snake venom protein omwaprin by total chemical synthesis and racemic protein crystallography"],"prefix":"10.1002","volume":"19","author":[{"given":"James R.","family":"Banigan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalyaneswar","family":"Mandal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Sawaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vilasak","family":"Thammavongsa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoni P. 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