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Deleted in split hand\/split foot 1 (Dss1\u2014Sem1 in budding yeast) is a highly multifunctional IDP associated with a range of protein complexes. However, it remains unknown if the different functions relate to different modified states. In this work, we show that <jats:italic>Schizosaccharomyces pombe<\/jats:italic> Dss1 is a substrate for casein kinase 2 in vitro, and we identify three phosphorylated threonines in its linker region separating two known disordered ubiquitin\u2010binding motifs. Phosphorylations of the threonines had no effect on ubiquitin\u2010binding but caused a slight destabilization of the C\u2010terminal \u03b1\u2010helix and mediated a direct interaction with the forkhead\u2010associated (FHA) domain of the RING\u2010FHA E3\u2010ubiquitin ligase defective in mitosis 1 (Dma1). The phosphorylation sites are not conserved and are absent in human Dss1. Sequence analyses revealed that the Txx(E\/D) motif, which is important for phosphorylation and Dma1 binding, is not linked to certain branches of the evolutionary tree. Instead, we find that the motif appears randomly, supporting the mechanism of ex nihilo evolution of novel motifs. In support of this, other threonine\u2010based motifs, although frequent, are nonconserved in the linker, pointing to additional functions connected to this region. We suggest that Dss1 acts as an adaptor protein that docks to Dma1 via the phosphorylated FHA\u2010binding motifs, while the C\u2010terminal \u03b1\u2010helix is free to bind mitotic septins, thereby stabilizing the complex. The presence of Txx(D\/E) motifs in the disordered regions of certain septin subunits may be of further relevance to the formation and stabilization of these complexes.<\/jats:p>","DOI":"10.1002\/pro.4733","type":"journal-article","created":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T15:50:12Z","timestamp":1689695412000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phosphorylation of <i>Schizosaccharomyces pombe<\/i> Dss1 mediates direct binding to the ubiquitin\u2010ligase Dma1 in vitro"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8264-0057","authenticated-orcid":false,"given":"Nina L.","family":"Jacobsen","sequence":"first","affiliation":[{"name":"Structural Biology and NMR Laboratory University of Copenhagen  Copenhagen N Denmark"},{"name":"REPIN University of Copenhagen  Copenhagen N Denmark"},{"name":"The Linderstr\u00f8m Lang Centre for Protein Science, 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