{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T14:31:17Z","timestamp":1759847477258,"version":"3.41.2"},"reference-count":51,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T00:00:00Z","timestamp":1694736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC108\u20102113\u2010M\u2010038\u2010002\u2010MY3"],"award-info":[{"award-number":["NSTC108\u20102113\u2010M\u2010038\u2010002\u2010MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004700","name":"Taipei Medical University","doi-asserted-by":"publisher","award":["DP2\u2010109\u201021121\u201001\u2010O\u201004\u201002","TMU106\u2010AE1\u2010B08"],"award-info":[{"award-number":["DP2\u2010109\u201021121\u201001\u2010O\u201004\u201002","TMU106\u2010AE1\u2010B08"]}],"id":[{"id":"10.13039\/501100004700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Protein oligomerization occurs frequently both in vitro and in vivo, with specific functionalities associated with different oligomeric states. The YqiC protein from <jats:italic>Salmonella<\/jats:italic> Typhimurium forms a homotrimer through its C\u2010terminal coiled\u2010coil domain, and the protein is closely linked to the colonization and invasion of the bacteria to the host cells. To elucidate the importance of the oligomeric state of YqiC in vivo and its relation with bacterial infection, we mutated crucial residues in YqiC's coiled\u2010coil region and confirmed the loss of trimer formation using chemical crosslinking and size exclusion chromatography coupled with multiple angle light scattering (SEC\u2010MALS) techniques. The <jats:italic>yqiC<\/jats:italic>\u2010knockout strain complemented with mutant YqiC showed significantly reduced colonization and invasion of <jats:italic>Salmonella<\/jats:italic> to host cells, demonstrating the critical role of YqiC oligomerization in bacterial pathogenesis. Furthermore, we conducted a protein\u2013protein interaction study of YqiC using a pulled\u2010down assay coupled with mass spectrometry analysis to investigate the protein's role in bacterial virulence. The results reveal that YqiC interacts with subunits of Complex II of the electron transport chain (SdhA and SdhB) and the \u03b2\u2010subunit of F<jats:sub>0<\/jats:sub>F<jats:sub>1<\/jats:sub>\u2010ATP synthase. These interactions suggest that YqiC may modulate the energy production of <jats:italic>Salmonella<\/jats:italic> and subsequently affect the assembly of crucial virulence factors, such as flagella. Overall, our findings provide new insights into the molecular mechanisms of YqiC's role in <jats:italic>S.<\/jats:italic> Typhimurium pathogenesis and suggest potential therapeutic targets for bacterial infections.<\/jats:p>","DOI":"10.1002\/pro.4749","type":"journal-article","created":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T14:45:39Z","timestamp":1691592339000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Salmonella<\/i><scp>YqiC<\/scp> exerts its function through an oligomeric state"],"prefix":"10.1002","volume":"32","author":[{"given":"Wei\u2010Chun","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wai\u2010Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Department 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Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1229-1138","authenticated-orcid":false,"given":"Tzu\u2010Wen","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei\u2010Ru","family":"Chang","sequence":"additional","affiliation":[{"name":"Division of Pediatric Gastroenterology and Hepatology, Department of Pediatrics, Shuang Ho Hospital Taipei Medical University  Taipei Taiwan"},{"name":"Department of Pediatrics, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1751-8354","authenticated-orcid":false,"given":"Yu\u2010Chu","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of 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