{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T21:27:38Z","timestamp":1771104458023,"version":"3.50.1"},"reference-count":67,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T00:00:00Z","timestamp":1695168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The pupal cuticle protein from <jats:italic>Aedes aegypti<\/jats:italic> (AaPC) inhibits dengue virus (DENV) infection; however, the underlying mechanism of this inhibition remains unknown. Here, we report that AaPC is an intrinsically disordered protein and interacts with domain I\/II of the DENV envelope protein via residues Asp59, Asp61, Glu71, Asp73, Ser75, and Asp80. AaPC can directly bind to and cause the aggregation of DENV, which in turn blocks virus infection during the virus\u2010cell fusion stage. AaPC may also influence viral recognition and attachment by interacting with human immune receptors DC\u2010SIGN and CD4. These findings enhance our understanding of the role of AaPC in mitigating viral infection and suggest that AaPC is a potential target for developing inhibitors or antibodies to control dengue virus infection.<\/jats:p>","DOI":"10.1002\/pro.4761","type":"journal-article","created":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T05:26:40Z","timestamp":1692422800000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Structural and functional characterization of <i>Aedes aegypti<\/i> pupal cuticle protein that controls dengue virus infection"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0635-7479","authenticated-orcid":false,"given":"Qingqing","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Biological Sciences National University of Singapore  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edem","family":"Gavor","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences National University of Singapore  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6577-6748","authenticated-orcid":false,"given":"Nikhil Kumar","family":"Tulsian","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences National University of Singapore  Singapore Singapore"},{"name":"Department of Biochemistry National University of Singapore  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingsong","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences National University of Singapore  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingsong","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences National University of Singapore  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2829-4878","authenticated-orcid":false,"given":"Yu Keung","family":"Mok","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences National University of Singapore  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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