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This process involves electron transfer through consecutive redox proteins that efficiently connect the inner membrane to the cell exterior. In this study, we isolated and characterized the quinone\u2010interacting membrane cytochrome <jats:italic>c<\/jats:italic> ImcH from <jats:italic>Geobacter sulfurreducens<\/jats:italic>, which is involved in the EET process to high redox potential acceptors. Spectroscopic and electrochemical studies show that ImcH hemes have low midpoint redox potentials, ranging from \u2212150 to \u2212358\u2009mV, and connect the oxidation of the quinol\u2010pool to EET, transferring electrons to the highly abundant periplasmic cytochrome PpcA with higher affinity than to its homologues. Despite the larger number of hemes and transmembrane helices, the ImcH structural model has similarities with the NapC\/NirT\/NrfH superfamily, namely the presence of a quinone\u2010binding site on the P\u2010side of the membrane. In addition, the first heme, likely involved on the quinol oxidation, has apparently an unusual His\/Gln coordination. Our work suggests that ImcH is electroneutral and transfers electrons and protons to the same side of the membrane, contributing to the maintenance of a proton motive force and playing a central role in recycling the menaquinone pool.<\/jats:p>","DOI":"10.1002\/pro.4796","type":"journal-article","created":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T03:14:30Z","timestamp":1696216470000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Characterization of the inner membrane cytochrome <scp>ImcH<\/scp> from <i>Geobacter<\/i> reveals its importance for extracellular electron transfer and energy conservation"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9952-8515","authenticated-orcid":false,"given":"Andreia I.","family":"Pimenta","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier Universidade Nova de Lisboa  Oeiras Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1511-3732","authenticated-orcid":false,"given":"Catarina M.","family":"Paquete","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier Universidade Nova de Lisboa  Oeiras Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3760-5180","authenticated-orcid":false,"given":"Leonor","family":"Morgado","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, NOVA School of Science and Technology Universidade NOVA de Lisboa  Caparica Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology Universidade NOVA de Lisboa  Caparica Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0243-3788","authenticated-orcid":false,"given":"Marcus J.","family":"Edwards","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Essex  Colchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6234-1914","authenticated-orcid":false,"given":"Thomas A.","family":"Clarke","sequence":"additional","affiliation":[{"name":"Centre for Molecular and Structural Biochemistry, School of Biological Sciences University of East Anglia  Norwich UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1136-809X","authenticated-orcid":false,"given":"Carlos A.","family":"Salgueiro","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, NOVA School of Science and Technology Universidade NOVA de Lisboa  Caparica Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology Universidade NOVA de Lisboa  Caparica Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3283-4520","authenticated-orcid":false,"given":"In\u00eas A. 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