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CSP uniformly coats the SPZ and plays a pivotal role in its immunobiology, in both the insect and the vertebrate hosts. Although CSP's N\u2010terminal domain (CSP<jats:sub>N<\/jats:sub>) has been reported to play an important role in multiple CSP functions, a thorough biophysical and structural characterization of CSP<jats:sub>N<\/jats:sub> is currently lacking. Here, we present an alternative method for the recombinant production and purification of CSP<jats:sub>N<\/jats:sub> from <jats:italic>Plasmodium falciparum<\/jats:italic> (PfCSP<jats:sub>N<\/jats:sub>), which provides pure, high\u2010quality protein preparations with high yields. 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