{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T07:43:33Z","timestamp":1786866213551,"version":"build-2736575974"},"reference-count":75,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T00:00:00Z","timestamp":1707955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The prevailing model of steroid hormone nuclear receptor function assumes ligand\u2010induced homodimer formation followed by binding to DNA hormone response elements (HREs). This model has been challenged by evidence showing that the glucocorticoid receptor (GR) forms tetramers upon ligand and DNA binding, which then drive receptor\u2010mediated gene transactivation and transrepression. GR and the closely\u2010related mineralocorticoid receptors (MR) interact to transduce corticosteroid hormone signaling, but whether they share the same quaternary arrangement is unknown. Here, we used a fluorescence imaging technique, Number &amp; Brightness, to study oligomerization in a cell system allowing real\u2010time analysis of receptor\u2010DNA interactions. Agonist\u2010bound MR forms tetramers in the nucleoplasm and higher order oligomers upon binding to HREs. Antagonists form intermediate\u2010size quaternary arrangements, suggesting that large oligomers are essential for function. Divergence between MR and GR quaternary structure is driven by different functionality of known and new multimerization interfaces, which does not preclude formation of heteromers. Thus, influencing oligomerization may be important to selectively modulate corticosteroid signaling.<\/jats:p>","DOI":"10.1002\/pro.4890","type":"journal-article","created":{"date-parts":[[2023,12,31]],"date-time":"2023-12-31T04:18:50Z","timestamp":1703996330000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The mineralocorticoid receptor forms higher order oligomers upon\n                    <scp>DNA<\/scp>\n                    binding"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6623-2264","authenticated-orcid":false,"given":"Gregory","family":"Fettweis","sequence":"first","affiliation":[{"name":"Laboratory of Receptor Biology and Gene Expression National Cancer Institute, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas 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Neurociencias (IFIBYNE), CONICET\u2010Universidad de Buenos Aires Facultad de Ciencias Exactas y Naturales  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon L.","family":"Hager","sequence":"additional","affiliation":[{"name":"Laboratory of Receptor Biology and Gene Expression National Cancer Institute, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4324-2793","authenticated-orcid":false,"given":"Diego","family":"Alvarez de la Rosa","sequence":"additional","affiliation":[{"name":"Laboratory of Receptor Biology and Gene Expression National Cancer Institute, National Institutes of Health  Bethesda Maryland USA"},{"name":"Departmento de Ciencias M\u00e9dicas B\u00e1sicas and Instituto de Tecnolog\u00edas Biom\u00e9dicas Universidad de La Laguna  La Laguna 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