{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T05:53:55Z","timestamp":1779170035307,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T00:00:00Z","timestamp":1715817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011912","name":"Taipei Veterans General Hospital","doi-asserted-by":"publisher","award":["VGHUST113\u2010G4\u20101\u20101"],"award-info":[{"award-number":["VGHUST113\u2010G4\u20101\u20101"]}],"id":[{"id":"10.13039\/501100011912","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020950","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 112\u20102311\u2010B008\u2010001"],"award-info":[{"award-number":["NSTC 112\u20102311\u2010B008\u2010001"]}],"id":[{"id":"10.13039\/501100020950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Prolyl\u2010tRNA synthetase (ProRS), belonging to the family of aminoacyl\u2010tRNA synthetases responsible for pairing specific amino acids with their respective tRNAs, is categorized into two distinct types: the eukaryote\/archaeon\u2010like type (E\u2010type) and the prokaryote\u2010like type (P\u2010type). Notably, these types are specific to their corresponding cognate tRNAs. In an intriguing paradox, <jats:italic>Thermus thermophilus<\/jats:italic> ProRS (TtProRS) aligns with the E\u2010type ProRS but selectively charges the P\u2010type tRNA<jats:sup>Pro<\/jats:sup>, featuring the bacterium\u2010specific acceptor\u2010stem elements G72 and A73. This investigation reveals TtProRS's notable resilience to the inhibitor halofuginone, a synthetic derivative of febrifugine emulating Pro\u2010A76, resembling the characteristics of the P\u2010type ProRS. Furthermore, akin to the P\u2010type ProRS, TtProRS identifies its cognate tRNA through recognition of the acceptor\u2010stem elements G72\/A73, along with the anticodon elements G35\/G36. However, in contrast to the P\u2010type ProRS, which relies on a strictly conserved R residue within the bacterium\u2010like motif 2 loop for recognizing G72\/A73, TtProRS achieves this through a non\u2010conserved sequence, RTR, within the otherwise non\u2010interacting eukaryote\u2010like motif 2 loop. This investigation sheds light on the adaptive capacity of a typically conserved housekeeping enzyme to accommodate a novel substrate.<\/jats:p>","DOI":"10.1002\/pro.5028","type":"journal-article","created":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T05:19:15Z","timestamp":1715923155000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Adaptive evolution: Eukaryotic enzyme's specificity shift to a bacterial substrate"],"prefix":"10.1002","volume":"33","author":[{"given":"Emi","family":"Latifah","sequence":"first","affiliation":[{"name":"Department of Life Sciences National Central University  Taoyuan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Indira Rizqita","family":"Ivanesthi","sequence":"additional","affiliation":[{"name":"Department of Life Sciences National Central University  Taoyuan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Kuan","family":"Tseng","sequence":"additional","affiliation":[{"name":"Graduate Institute of Statistics National Central University  Taoyuan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hung\u2010Chuan","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Taichung Veterans General Hospital  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7104-1307","authenticated-orcid":false,"given":"Chien\u2010Chia","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Life Sciences National Central University  Taoyuan 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